PMID: 2121946Nov 1, 1990Paper

Interpretation of IgG subclass values: a comparison of two assays

The Journal of Pediatrics
C MeissnerD M Ambrosino

Abstract

Because we have noted discordant results in the measurement of IgG subclass concentrations by means of a widely available commercial radial immunodiffusion (RID) kit in comparison with an enzyme-linked immunosorbent assay (ELISA) developed at the Centers for Disease Control (CDC), we conducted in a blinded manner a comparison of the two assays, using sera from 48 healthy children. The correlation coefficients between the assays were 0.92, 0.82, 0.93, and 0.86 for the IgG1, IgG2, IgG3, and IgG4 assays, respectively. However, the RID assay assigned lower values for IgG1 and IgG4 determinations than the ELISA did. Furthermore, the "normal lower range values" provided by the RID assay were higher for each IgG subclass. When the sera from the healthy control subjects were analyzed with the RID assay, 12 (25%) of 48 subjects had values below the normal range for at least one subclass measurement. In contrast, with the CDC ELISA, all values were within the 95% confidence limits determined for the CDC ELISA. We suggest that age-specific normal limits be established with the use of sera from many healthy subjects for any assay measuring IgG subclass concentrations. As new groups of immunodeficiencies are defined and potential therapies ...Continue Reading

References

Jan 1, 1979·Acta paediatrica Scandinavica·V A Oxelius
Jan 21, 1988·Journal of Immunological Methods·C M BlackC B Reimer
Dec 18, 1986·The New England Journal of Medicine·D M GranoffR S Daum
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Citations

Mar 1, 1994·Archives of Disease in Childhood·P H HoegerG Haeuser
Mar 16, 2016·European Archives of Oto-rhino-laryngology : Official Journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : Affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery·Haitham Odat, Mohannad Alqudah
Dec 1, 1991·The Journal of Pediatrics·D M AmbrosinoG M Carlone
Jul 30, 2002·Pediatric Allergy and Immunology : Official Publication of the European Society of Pediatric Allergy and Immunology·Cleonir de Moraes LuiAnete Sevciovic Grumach
Nov 1, 1991·Clinical Immunology and Immunopathology·L A HansonP Brandtzaeg
May 1, 1995·Annals of Clinical Biochemistry·M PressacP Aymard

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