Abstract
Time-resolved fluorescence of 3-methylbenzimidazole (m(3)B) was used to study stacking interaction between base moieties in di-, tri- and tetra-phosphate analogues of 3-methylbenzimidazolyl(5'-5')guanosine (m(3)Bp( n )G, n = 2, 3, 4), using 5'-triphosphate of 3-methylbenzimidazole riboside (m(3)BTP) as reference. Fluorescence intensity decays of all compounds cannot be satisfactory fitted with single-exponential function. Although an increase of a number of exponents led to better fits, interpretation of the individual exponential terms, i.e. pre-exponential amplitudes and fluorescence lifetimes, cannot be adequately characterized. We show that these fluorescence decays are best fitted by power-like function derived from physically justified distribution of the fluorescence lifetimes, and characterized by the mean value of the excited-state lifetime and relative variance of lifetime fluctuations around the mean value. The latter led to the parameter of heterogeneity and number of decay paths, which depend on the factors responsible for non-radiative decay of the excited state, including base-base stacking interaction. This was studied by means of changes of temperature and the number of phosphate groups in dinucleotides. It was...Continue Reading
References
Oct 3, 1989·Biochemistry·S E CarberryD J Goss
Jan 1, 1988·Progress in Nucleic Acid Research and Molecular Biology·N Sonenberg
Oct 1, 1983·Analytical Biochemistry·P Zamecnik
Mar 11, 1980·Nucleic Acids Research·Y NishimuraT Hata
Jan 12, 1996·Science·D A Dougherty
Jun 13, 1997·Cell·J MarcotrigianoS K Burley
Dec 13, 1997·Biochimica Et Biophysica Acta·Z WieczorekR Stolarski
Aug 18, 1999·Proceedings of the National Academy of Sciences of the United States of America·J P Gallivan, D A Dougherty
Oct 6, 2000·Physical Review Letters·G Wilk, Z Wlodarczyk
Oct 29, 2000·Advances in Virus Research·Y Furuichi, A J Shatkin
Jan 13, 2001·European Biophysics Journal : EBJ·E Błachut-OkrasińskaJ M Antosiewicz
May 9, 2001·Biochimica Et Biophysica Acta·G StoychevD Shugar
Mar 7, 2002·The Biochemical Journal·Koji TomooKin-ichiro Miura
Jun 11, 2002·Journal of Molecular Biology·Anna NiedzwieckaRyszard Stolarski
Aug 9, 2002·The Journal of Biological Chemistry·Christophe BiotMarianne Rooman
Feb 13, 2003·European Biophysics Journal : EBJ·M DługoszJ M Antosiewicz
Apr 15, 2003·Journal of Molecular Biology·Koji TomooToshimasa Ishida
Jun 28, 2003·Biophysical Journal·Jakub Włodarczyk, Borys Kierdaszuk
Aug 29, 2003·Biophysical Journal·Katarzyna RuszczynskaRyszard Stolarski
Oct 24, 2003·The Journal of Biological Chemistry·Guanghui HuFlorante A Quiocho
Dec 16, 2003·Angewandte Chemie·Melanie A O'NeillAhmed H Zewail
Apr 27, 2004·Nucleic Acids Research·T-J SuD T F Dryden
Aug 4, 2004·Biochemistry·John M Jean, Kathleen B Hall
Sep 16, 2004·Journal of the American Chemical Society·Melanie A O'Neill, Jacqueline K Barton
Oct 11, 2005·Gene·Marilyn Kozak