Abstract
An incubation time of 24 h at 35 to 38 degrees C is recommended for the optimal performance of MRSASelect (Bio-Rad) chromogenic screening agar. An additional 24 h is required to capture slow-growing methicillin-resistant Staphylococcus aureus (MRSA). However, the normal hours of operation for most laboratories cannot reliably accommodate 24-h interpretation intervals. Daily agar plate interpretations are more likely to occur around 18 h and 42 h of incubation, which may compromise the performance of the chromogenic agar and negatively impact patient infection control efforts. In order to validate the importance of stringent incubation times to plate performance, we evaluated MRSASelect medium at controlled intervals of 24 and 48 h of incubation, using clinical MRSA-screening swabs. A total of 1,071 MRSA-positive and 2,733 MRSA-negative cultures were selected for analysis. Compared to 48-h-incubation results, the sensitivity and specificity of MRSASelect at 24 h were 98.3% and 98.2%, respectively. Only 19 of 1,071 (1.8%) MRSA-positive isolates required 48 h for detection. Holding 24-h negative plates an additional 24 h resulted in the workup of 253 (6.6%) pink, yet non-MRSA, colonies. The 24-h positive and negative predictive va...Continue Reading
References
Jan 1, 1997·The Journal of Hospital Infection·E M JonesA P MacGowan
Jun 29, 2001·The Journal of Hospital Infection·R W SmythB Hoffman
Jun 6, 2003·Infection Control and Hospital Epidemiology : the Official Journal of the Society of Hospital Epidemiologists of America·Carlene A MutoUNKNOWN SHEA
Oct 9, 2004·Journal of Clinical Microbiology·John D PerryF Kate Gould
Feb 4, 2006·Journal of Clinical Microbiology·Luba StoakesZafar Hussain
May 13, 2006·International Journal of Antimicrobial Agents·Sana Ben NsiraRoland Leclercq
Oct 20, 2006·Journal of Clinical Microbiology·Lisa LouieAndrew E Simor
Nov 24, 2006·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·I NahimanaD S Blanc
Mar 22, 2007·Journal of Medical Microbiology·I H M van LooA G M Buiting
Mar 22, 2007·Journal of Medical Microbiology·Abdessalam CherkaouiJacques Schrenzel
Citations
Jul 20, 2011·Surgical Infections·Charles E EdmistonGary R Seabrook
Nov 9, 2012·Journal of Clinical Microbiology·Gerald A DenysCarol M Wissel
Apr 16, 2010·Journal of Clinical Microbiology·Stefan RiedelKaren C Carroll
Apr 16, 2010·Journal of Clinical Microbiology·C WendtA M Fahr
Feb 3, 2016·Surgical Infections·Charles E EdmistonDavid Leaper
Apr 3, 2012·The Journal of Hospital Infection·K MorrisM H Wilcox
May 26, 2011·Anaesthesia·S MalhotraS M Yentis
Mar 8, 2013·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·C GarzoniUNKNOWN AST Infectious Diseases Community of Practice
Jul 23, 2015·Military Medical Research·Volker MicheelHagen Frickmann
Mar 4, 2014·Journal of Dairy Science·K M Cicconi-HoganY H Schukken
Apr 17, 2015·Annals of Clinical Microbiology and Antimicrobials·Roli KarguptaShramik Sengupta
Jan 1, 2016·Journal of Clinical Microbiology·Matthew L FaronNathan A Ledeboer
May 3, 2014·Annals of Laboratory Medicine·Yirang LeeKyu Man Lee
Jun 15, 2013·Hemodialysis International·Lee Ying YeohSay Tat Ooi
Jun 24, 2016·Microbiology Spectrum·N Esther Babady