PMID: 3746894Jan 1, 1986Paper

Interpretation of steady-state current-voltage curves: consequences and implications of current subtraction in transport studies

The Journal of Membrane Biology
M R Blatt

Abstract

A problem often confronted in analyses of charge-carrying transport processes in vivo lies in identifying porter-specific component currents and their dependence on membrane potential. Frequently, current-voltage (I-V)--or more precisely, difference-current-voltage (dI-V)--relations, both for primary and for secondary transport processes, have been extracted from the overall membrane current-voltage profiles by subtracting currents measured before and after experimental manipulations expected to alter the porter characteristics only. This paper examines the consequences of current subtraction within the context of a generalized kinetic carrier model for Class I transport mechanisms (U.-P. Hansen, D. Gradmann, D. Sanders and C.L. Slayman, 1981, J. Membrane Biol. 63:165-190). Attention is focused primarily on dI-V profiles associated with ion-driven secondary transport for which external solute concentrations usually serve as the experimental variable, but precisely analogous results and the same conclusions are indicated in relation to studies of primary electrogenesis. The model comprises a single transport loop linking n (3 or more) discrete states of a carrier 'molecule.' State transitions include one membrane charge-transpor...Continue Reading

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Citations

May 1, 1990·The Journal of Membrane Biology·S R McCullochN A Walker
Jun 15, 2007·The Journal of Membrane Biology·Sabrina GazzarriniAnna Moroni
Jan 1, 1988·Progress in Biophysics and Molecular Biology·D W Hilgemann
May 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·M R Blatt, C L Slayman
Jan 1, 1989·Annals of the New York Academy of Sciences·D Gradmann
Sep 6, 2011·Biophysical Journal·Dietrich GradmannPeter Hegemann
Jan 1, 1987·The Journal of Membrane Biology·M R BlattC L Slayman

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