Interpreting in vivo calcium signals from neuronal cell bodies, axons, and dendrites: a review

Neurophotonics
Farhan Ali, Alex C Kwan

Abstract

Calcium imaging is emerging as a popular technique in neuroscience. A major reason is that intracellular calcium transients are reflections of electrical events in neurons. For example, calcium influx in the soma and axonal boutons accompanies spiking activity, whereas elevations in dendrites and dendritic spines are associated with synaptic inputs and local regenerative events. However, calcium transients have complex spatiotemporal dynamics, and since most optical methods visualize only one of the somatic, axonal, and dendritic compartments, a straightforward inference of the underlying electrical event is typically challenging. We highlight experiments that have directly calibrated in vivo calcium signals recorded using fluorescent indicators against electrophysiological events. We address commonly asked questions such as: Can calcium imaging be used to characterize neurons with high firing rates? Can the fluorescent signal report a decrease in spiking activity? What is the evidence that calcium transients in subcellular compartments correspond to distinct presynaptic axonal and postsynaptic dendritic events? By reviewing the empirical evidence and limitations, we suggest that, despite some caveats, calcium imaging is a vers...Continue Reading

References

May 1, 1992·The Journal of Physiology·E Neher, G J Augustine
Apr 6, 1990·Science·W DenkW W Webb
Jan 1, 1987·Annual Review of Neuroscience·G J AugustineS J Smith
May 24, 1994·Proceedings of the National Academy of Sciences of the United States of America·H Markram, B Sakmann
Mar 1, 1997·Trends in Neurosciences·G StuartM Häusser
Oct 3, 1999·Nature Biotechnology·R Y Tsien
Oct 20, 1999·Nature Neuroscience·F HelmchenD W Tank
Dec 10, 1999·Proceedings of the National Academy of Sciences of the United States of America·M E LarkumB Sakmann
Apr 5, 2000·Nature·J SchillerY Schiller
Aug 10, 2000·Proceedings of the National Academy of Sciences of the United States of America·C L CoxK Svoboda
Jan 3, 2001·Nature Neuroscience·G J Stuart, M Häusser
Feb 13, 2001·Journal of Neurophysiology·P VetterM Häusser
Oct 15, 2003·Neuron·George J AugustineKeiko Tanaka
Nov 5, 2003·Nature Biotechnology·Warren R ZipfelWatt W Webb
Dec 14, 2004·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Jack Waters, Fritjof Helmchen
Mar 19, 2005·Science·Helmut J Koester, Daniel Johnston
Sep 15, 2005·Proceedings of the National Academy of Sciences of the United States of America·Jason N D KerrFritjof Helmchen
Feb 16, 2008·The Journal of Physiology·Yexica ApontePeter Jonas
Apr 17, 2008·The Journal of Physiology·Erwin Neher
Sep 27, 2008·Neuron·Michael J Higley, Bernardo L Sabatini
Oct 24, 2008·Frontiers in Neural Circuits·Laurent Moreaux, Gilles Laurent
Jan 20, 2009·Nature·Masanori MurayamaMatthew E Larkum
Jul 22, 2009·Biophysical Journal·Joshua T VogelsteinLiam Paninski
Oct 27, 2009·Current Opinion in Neurobiology·Benjamin F Grewe, Fritjof Helmchen
Nov 18, 2009·Proceedings of the National Academy of Sciences of the United States of America·Masanori Murayama, Matthew E Larkum
Apr 30, 2010·Nature·Hongbo JiaArthur Konnerth
Jun 28, 2011·Nature·Xiaowei ChenArthur Konnerth

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Citations

Oct 31, 2019·Journal of Neurophysiology·Brandon K LaPalloMarie-Claude Perreault
Mar 29, 2020·Sensors·Elena von MolitorTiziana Cesetti
Nov 14, 2020·International Journal of Molecular Sciences·Malwina LisekTomasz Boczek
Apr 13, 2021·Frontiers in Neuroscience·Austin NeugornetPavel Ivanovich Ortinski
May 1, 2021·Micromachines·Elles A L RaaijmakersRegina Luttge
Jul 6, 2021·Frontiers in Neuroscience·Silviu-Vasile Bodea, Gil Gregor Westmeyer
Jun 14, 2021·Journal of Neuroscience Methods·Gerard Joey Broussard, Leopoldo Petreanu
Oct 2, 2021·The International Journal of Biostatistics·Hermine BierméChristine Georgelin

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Methods Mentioned

BETA
transgenic
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imaging techniques
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