Interrelation between Tween and the membrane properties and high pressure tolerance of Lactobacillus plantarum

BMC Microbiology
Dominik ReitermayerRudi F Vogel

Abstract

Tween® 80 is a frequently used supplement of media for the cultivation of lactic acid bacteria. We investigated its effect on the cell physiology and stress tolerance of Lactobacillus (L.) plantarum. Data on the transcriptomic response to Tween 80 supplementation and its effects on cellular fatty acid profiles and growth characteristics are compared with data characterizing the effect of Tween 80, other Tween types and free fatty acids on the high hydrostatic pressure (HHP) tolerance of L. plantarum strain TMW 1.708. These include effects on cell viability, sub-lethal injury, metabolic activity, protein release and propidium iodide uptake. Tween 80 caused the downregulation of fatty acid biosynthesis and an increase in oleic acid and cyclopropane fatty acid levels in the cell membrane. Tween 20, Tween 80 and free oleic acid, but not Tween 40, Tween 60 and other free fatty acids, conferred resistance against HHP. Tween 80 diminished pressure-induced loss of metabolic activity, protein release and uptake of propidium iodide. However, loss of cell viability exceeded by far membrane permeabilization, suggesting that membrane permeabilization, which has frequently been postulated as a major factor in HHP inactivation of microbes, is...Continue Reading

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Citations

Feb 19, 2021·Journal of Bioscience and Bioengineering·Thi-Lan-Phuong NguyenCatherine Béal
Sep 24, 2021·Journal of Industrial Microbiology & Biotechnology·Syed S ShahMargaret L Britz

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Methods Mentioned

BETA
light absorption spectrometry

Software Mentioned

Rockhopper
AxioVS40 V
Sigmaplot

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