Interrelationships between Fatty Acid Composition, Staphyloxanthin Content, Fluidity, and Carbon Flow in the Staphylococcus aureus Membrane

Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry
Kiran B TiwariBrian J Wilkinson

Abstract

Fatty acids play a major role in determining membrane biophysical properties. Staphylococcus aureus produces branched-chain fatty acids (BCFAs) and straight-chain saturated fatty acids (SCSFAs), and can directly incorporate exogenous SCSFAs and straight-chain unsaturated fatty acids (SCUFAs). Many S. aureus strains produce the triterpenoid pigment staphyloxanthin, and the balance of BCFAs, SCSFAs and staphyloxanthin determines membrane fluidity. Here, we investigated the relationship of fatty acid and carotenoid production in S. aureus using a pigmented strain (Pig1), its carotenoid-deficient mutant (Pig1ΔcrtM) and the naturally non-pigmented Staphylococcus argenteus that lacks carotenoid biosynthesis genes and is closely related to S. aureus. Fatty acid compositions in all strains were similar under a given culture condition indicating that staphyloxanthin does not influence fatty acid composition. Strain Pig1 had decreased membrane fluidity as measured by fluorescence anisotropy compared to the other strains under all conditions indicating that staphyloxanthin helps maintain membrane rigidity. We could find no evidence for correlation of expression of crtM and fatty acid biosynthesis genes. Supplementation of medium with gluc...Continue Reading

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Citations

Apr 24, 2019·Antimicrobial Agents and Chemotherapy·Selvi C ErsoyArnold S Bayer
May 6, 2020·Journal of Bacteriology·Zachary DeMarsJeffrey L Bose
Feb 26, 2019·BioMed Research International·Hannah Braungardt, Vineet K Singh
Apr 17, 2021·Biochimica Et Biophysica Acta. Molecular and Cell Biology of Lipids·Gerson-Dirceu LópezChiara Carazzone

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