PMID: 2503588Sep 1, 1989Paper

Interrelationships between glucose metabolism, energy state, and the cytosolic free calcium concentration in cortical synaptosomes from the guinea pig

Journal of Neurochemistry
R A KauppinenH Komulainen

Abstract

The stoichiometries of glycolysis and pyruvate oxidation were determined in cortical synaptosomes under varying rates of ATP consumption. Glycolysis was measured by using D-3-[3H]glucose as a marker and pyruvate oxidation by using D-3,4-[14C]glucose, which has to be metabolized to 1-[14C]pyruvate before being decarboxylated by the pyruvate dehydrogenase complex of intrasynaptosomal mitochondria. Cytosolic free Ca2+ concentration [( Ca2+]c) was determined in parallel and was manipulated by using EGTA in the incubation. The results show that in nonstimulated synaptosomes glycolysis and pyruvate oxidation are tightly coupled and stoichiometric. In the absence of Ca2+, when [Ca2+]c drops from 260 nM to 40 nM, glucose utilization increases, following the increase in energy demand, which has been shown to be due to elevated Na+ cycling. KCl depolarization, veratridine, and a mitochondrial uncoupler, carbonyl cyanide m-chlorophenylhydrazone, all stimulate glycolysis and pyruvate oxidation stoichiometrically, independently of the presence of external Ca2+. A rise in [Ca2+]c, therefore, is not required to regulate mitochondrial pyruvate metabolism. It is concluded that synaptosomes exhibit a high degree of respiratory control, that they...Continue Reading

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Citations

Apr 18, 2002·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·José L Soengas, Manuel Aldegunde
Oct 1, 1992·Journal of Basic and Clinical Physiology and Pharmacology·D WermelskirchenT Peters
Dec 22, 2011·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Gerald A Dienel
Mar 24, 2011·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Silvia MangiaSusan J Vannucci
Dec 18, 2019·PloS One·Dimitris G PatsatzisS Mani Sarathy
Dec 20, 2018·Physiological Reviews·Gerald A Dienel
Sep 13, 1991·Biochimica Et Biophysica Acta·H T McMahon, D G Nicholls

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