Interrogation of Cellular Innate Immunity by Diamond-Nanoneedle-Assisted Intracellular Molecular Fishing

Nano Letters
Zixun WangPeng Shi

Abstract

Understanding intracellular signaling cascades and network is one of the core topics in modern biology. Novel tools based on nanotechnologies have enabled probing and analyzing intracellular signaling with unprecedented sensitivity and specificity. In this study, we developed a minimally invasive method for in situ probing specific signaling components of cellular innate immunity in living cells. The technique was based on diamond-nanoneedle arrays functionalized with aptamer-based molecular sensors, which were inserted into cytoplasmic domain using a centrifugation controlled process to capture molecular targets. Simultaneously, these diamond-nanoneedles also facilitated the delivery of double-strand DNAs (dsDNA90) into cells to activate the pathway involving the stimulator of interferon genes (STING). We showed that the nanoneedle-based biosensors can be successfully utilized to isolate transcriptional factor, NF-κB, from intracellular regions without damaging the cells, upon STING activation. By using a reversible protocol and repeated probing in living cells, we were able to examine the singling dynamics of NF-κB, which was quickly translocated from cytoplasm to nucleus region within ∼40 min of intracellular introduction of...Continue Reading

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Citations

Apr 13, 2017·Nanoscale·Nina Buch-MånsonKaren L Martinez
Dec 13, 2016·Chemical Society Reviews·Xianfeng Chen, Wenjun Zhang
Jul 22, 2017·ACS Sensors·Ciro Chiappini
Mar 24, 2018·Annual Review of Analytical Chemistry·Allister F McGuireBianxiao Cui
Oct 20, 2018·Current Drug Delivery·Dong HuangYuanyu Huang
Jan 17, 2020·Advanced Materials·Stuart G HigginsMolly M Stevens
Aug 28, 2020·Advanced Materials·Yaping ChenRoey Elnathan
Aug 25, 2021·Nature Protocols·Ciro ChiappiniRoey Elnathan
Jun 11, 2020·Nano Letters·Yuan WangPeng Shi

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