Abstract
Interruptin B has been isolated from Cyclosorus terminans, however, its pharamcological effect has not been fully identified. In the present study, the effects of interruptin B, from C. terminans, on brown adipocyte differentiation and glucose uptake in adipose‑derived stem cells (ASCs) were investigated. The results revealed that interruptin B dose‑dependently enhanced the adipogenic differentiation of ASCs, with an induction in the mRNA expression levels of peroxisome proliferator‑activated receptor (PPAR)‑α and PPAR‑γ. In addition, interruptin B efficiently increased the number and the membrane potential of mitochondria and upregulated the mRNA expression levels of uncoupling protein (UCP)‑1 and cyclooxygenase (COX)‑2, which are all predominantly expressed in brown adipocytes. Interruptin B increased glucose consumption in differentiated ASCs, accompanied by the upregulation in the mRNA expression levels of glucose transporter (GLUT)‑1 and GLUT‑4. The computational analysis of molecular docking, a luciferase reporter assay and surface plasmon resonance confirmed the marked binding affinity of interruptin B to PPAR‑α and PPAR‑γ (K(D) values of 5.32 and 0.10 µm, respectively). To the best of our knowledge, the present study is...Continue Reading
References
Nov 24, 1995·The Journal of Biological Chemistry·L TeboulP A Grimaldi
Feb 29, 2000·Archives of Family Medicine·J HauptmanK R Segal
Jan 13, 2004·Physiological Reviews·Barbara Cannon, Jan Nedergaard
Oct 4, 2005·Lancet·David W Haslam, W Philip T James
Oct 19, 2005·Appetite·Jason C G Halford
Nov 25, 2005·Diabetes & Vascular Disease Research : Official Journal of the International Society of Diabetes and Vascular Disease·Chetan PatelDarren K McGuire
Jan 20, 2006·Journal of Medicinal Chemistry·Woody ShermanRamy Farid
Mar 3, 2006·The Journal of Clinical Investigation·Robert K SempleStephen O'Rahilly
May 10, 2006·The Journal of Pharmacology and Experimental Therapeutics·Takao FujimuraSeitaro Mutoh
Aug 8, 2007·Annals of Internal Medicine·George A DiamondSanjay Kaul
Dec 22, 2007·Drug Safety : an International Journal of Medical Toxicology and Drug Experience·Theodosios D FilippatosMoses S Elisaf
Oct 3, 2008·Journal of Dermatological Science·Won-Serk KimJong-Hyuk Sung
Apr 21, 2009·Life Sciences·Sun-Sil ChoiJe-Tae Woo
Apr 29, 2009·Journal of Computational Chemistry·Garrett M MorrisArthur J Olson
Jun 12, 2009·Lancet·Robert R HenryMatthias Herz
Sep 25, 2009·The Journal of Nutritional Biochemistry·Kae Won ChoYoung-Cheul Kim
Jan 1, 2008·Current Chemical Genomics·Vandana Kumari, Chenglong Li
Jul 27, 2010·Archives of Internal Medicine·Steven E Nissen, Kathy Wolski
Sep 3, 2010·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Jalees Rehman
Dec 1, 2010·Journal of Chemical Theory and Computation·Emilio GallicchioRonald M Levy
Jan 12, 2011·Biochemical Pharmacology·Sun-Sil ChoiJe-Tae Woo
Nov 22, 2012·Best Practice & Research. Clinical Endocrinology & Metabolism·Gema Medina-Gómez
Nov 25, 2014·Expert Opinion on Therapeutic Patents·Ichiro Takada, Makoto Makishima
Sep 9, 2015·JAMA : the Journal of the American Medical Association·David M Nathan