Interspecific germ cell transplantation: a new light in the conservation of valuable Balkan trout genetic resources?

Fish Physiology and Biochemistry
Jelena LujićÁkos Horváth

Abstract

Interspecific transplantation of germ cells from the brown trout Salmo trutta m. fario and the European grayling Thymallus thymallus into rainbow trout Oncorhynchus mykiss recipients was carried out in order to improve current practices in conservation of genetic resources of endangered salmonid species in the Balkan Peninsula. Current conservation methods mainly include in situ efforts such as the maintenance of purebred individuals in isolated streams and restocking with purebred fingerlings; however, additional ex situ strategies such as surrogate production are needed. Steps required for transplantation such as isolation of high number of viable germ cells and fluorescent labeling of germ cells which are to be transplanted have been optimized. Isolated and labeled brown trout and grayling germ cells were intraperitoneally transplanted into 3 to 5 days post hatch rainbow trout larvae. Survival of the injected larvae was comparable to the controls. Sixty days after transplantation, fluorescently labeled donor cells were detected within the recipient gonads indicating successful incorporation of germ cells (brown trout spermatogonia and oogonia-27%; grayling spermatogonia-28%; grayling oogonia-23%). PCR amplification of donor ...Continue Reading

References

Oct 21, 1999·Biology of Reproduction·I DobrinskiR L Brinster
Dec 26, 2001·Biology of Reproduction·Ali HonaramoozIna Dobrinski
Sep 18, 2007·Science·Tomoyuki OkutsuGoro Yoshizaki
Mar 13, 2010·Development·Goro YoshizakiTomoyuki Okutsu
Jun 15, 2010·Comparative Biochemistry and Physiology. Part D, Genomics & Proteomics·Goro YoshizakiYutaka Takeuchi
Mar 29, 2011·Molecular Phylogenetics and Evolution·G PustovrhA Snoj
Jan 16, 2013·Proceedings of the National Academy of Sciences of the United States of America·Seungki LeeGoro Yoshizaki
Jun 25, 2013·Molecular Reproduction and Development·Shinya ShikinaGoro Yoshizaki
Nov 30, 2013·International Journal of Molecular Sciences·Patrick BerrebiAlain J Crivelli
Jan 7, 2015·Theriogenology·Martin PšeničkaIevgeniia Gazo
Jun 19, 2016·General and Comparative Endocrinology·Juan F AsturianoÁkos Horváth
Apr 26, 2017·Cryobiology·Jelena LujićÁkos Horváth

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Citations

Jun 26, 2018·Reproduction in Domestic Animals = Zuchthygiene·Jelena LujićÁkos Horváth
Feb 18, 2019·Biology of Reproduction·Pierre Comizzoli, William V Holt
Apr 19, 2019·PloS One·Roman FraněkÁkos Horváth
Apr 20, 2019·Animals : an Open Access Journal From MDPI·Abdul Rasheed BalochMartin Pšenička
Nov 11, 2020·Scientific Reports·Nicola RiversPeter Temple-Smith

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Methods Mentioned

BETA
biopsy
PCR
SGA
dissection
transgenic

Software Mentioned

BLAST
ImageJ
Primer
STATISTICA

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