Jan 28, 2014

Interspecific small molecule interactions between clinical isolates of Pseudomonas aeruginosa and Staphylococcus aureus from adult cystic fibrosis patients

PloS One
Alexandre FugèreFrançois Malouin

Abstract

Pseudomonas aeruginosa and Staphylococcus aureus are the most prevalent pathogens in airway infections of cystic fibrosis (CF) patients. We studied how these pathogens coexist and interact with each other. Clinical isolates of both species were retrieved from adult CF patients. Culture supernatants from 63 P. aeruginosa isolates triggered a wide range of biofilm-stimulatory activities when added to the culture of a control S. aureus strain. The extent of biofilm formation by S. aureus was positively correlated to the levels of the 2-alkyl-4-(1H)-quinolones (AQs) Pseudomonas Quinolone Signal (PQS) and 2-heptyl-4-hydroxy quinoline N-oxide (HQNO) produced by the P. aeruginosa isolates. Supernatants from P. aeruginosa isogenic mutants deficient in PQS and HQNO production stimulated significantly less biofilm formation by S. aureus than that seen with the parental strain PA14. When studying co-isolated pairs of P. aeruginosa and S. aureus retrieved from patients showing both pathogens, P. aeruginosa supernatants stimulated less biofilm production by the S. aureus counterparts compared to that observed using the control S. aureus strain. Accordingly, some P. aeruginosa isolates produced low levels of exoproducts and also some of the ...Continue Reading

  • References57
  • Citations20

References

Mentioned in this Paper

Gram-Positive Bacteria
Neuro-Oncological Ventral Antigen 2
Pseudomonas Infections
Surface Enhanced Laser Desorption Ionization Mass Spectrometry
Biochemical Pathway
Pseudomonas aeruginosa (antigen)
Respiratory Tract Structure
Methicillin resistant Staphylococcus aureus (organism)
Endogenous Surfactants
Lung

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