Interstitial collagen synthesis and processing in human amnion: a property of the mesenchymal cells

Biology of Reproduction
M L Casey, P C MacDonald

Abstract

This study was conducted to evaluate the ontogeny and cellular site of interstitial collagen formation in amnion, the tissue that provides the tensile strength of the human fetal membranes. The levels of procollagen alpha 1(I), alpha 2(I), and alpha 1(III) subunit mRNAs and the specific activities of the enzymes prolyl 4-hydroxylase and lysyl hydroxylase were greatest in human amnion tissue early in gestation, decreasing rather abruptly after 12-14 wk gestation to a nadir that persisted to term. To evaluate these findings further, amnion epithelial and mesenchymal cells were separated by differential proteinase treatment. The freshly separated cells as well as epithelial and mesenchymal cells maintained in culture were evaluated to assess the cellular site of interstitial collagen synthesis. By Northern analysis of total RNA, large amounts of procollagens alpha 1(I), alpha 2(I), and alpha 1(III) mRNAs were found in mesenchymal cells (freshly separated and in culture), but only negligible amounts of these transcripts were detected in RNA of freshly separated or cultured epithelial cells. The level of prolyl 4-hydroxylase alpha-subunit mRNA in mesenchymal cells was somewhat greater than that in epithelial cells. Radiolabeled coll...Continue Reading

Citations

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Nov 5, 1997·The Journal of Clinical Endocrinology and Metabolism·M L Casey, P C MacDonald

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