PMID: 9435616Jan 22, 1998Paper

Interstitial exclusion of macromolecules studied by graded centrifugation of rat tail tendon

The American Journal of Physiology
K AuklandE M Renkin

Abstract

Mechanical compression of cartilage and tendon has been shown to expel fluid both from collagen fibrils and from the extrafibrillar space. As reported previously, albumin (Alb) concentration and colloid osmotic pressure in tendon fluid (TF) expelled by repeated centrifugations fell progressively at increasing centrifugation force (G = 600, 2,400, and 13,100), suggesting either molecular sieving in compressed tendon or mobilization of protein-free (excluded) fluid. The present experiments, including analysis of 51Cr-EDTA, aprotinin (Ap), Alb, immunoglobulin G (IgG), and hyaluronan (hyaluronic acid; HA) with molecular weight (MW) ranging from 341 to 5 x 10(6), strongly favored the exclusion hypothesis; the fraction of Alb, IgG, and HA-free fluid (excluded) was already 0.23-0.36 in the first centrifugate, increasing to 0.73-0.82 in the third. The corresponding numbers were, respectively, 0.11 and 0.43 for Ap (MW 6,500), and 0 and 0.08 for 51Cr-EDTA. These data, combined with calculated exclusion by collagen fibrils, proteoglycans, and HA, indicated that the first centrifugate was mainly derived from the extrafibrillar space, with increasing addition of macromolecular free intrafibrillar fluid in the second and third centrifugates,...Continue Reading

References

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Citations

Sep 15, 2001·American Journal of Physiology. Heart and Circulatory Physiology·K AuklandH Wiig
Oct 22, 2002·American Journal of Physiology. Heart and Circulatory Physiology·Helge WiigOlav Tenstad
Aug 10, 2010·American Journal of Physiology. Heart and Circulatory Physiology·H K BrekkeT Nedrebø
Jan 13, 2004·American Journal of Physiology. Heart and Circulatory Physiology·Helge WiigPer Ole Iversen
Apr 25, 2000·American Journal of Physiology. Heart and Circulatory Physiology·H WiigO Tenstad
May 27, 2003·The Journal of Physiology·Helge WiigJoel L Bert
Jun 3, 2021·International Journal of Molecular Sciences·Iris RibitschFlorien Jenner

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