Interstitial fluid analysis for assessment of organ function

Clinical Transplantation
Simona C BaicuKelvin G M Brockbank

Abstract

Evaluation methods are required for non-heart-beating donor (NHBD) kidneys to ensure the success of transplantation. In this study, the microdialysis technique was employed for the ex-vivo assessment of hypothermically preserved NHBD kidney function. Microdialysis probes were placed in the renal cortex of 2 h warm ischaemic porcine kidneys to monitor interstitial pyruvate dynamics during hypothermic machine perfusion with perfusate containing 29.4 mM fructose-1,6-diphosphate (FDP). The presence of exogenous FDP in the perfusate induced no changes in the renal flow rate and vascular resistance, renal artery effluent biochemistry, or pyruvate concentration relative to untreated control kidneys. Significant increases in pyruvate production (P < 0.05), however, were observed after 12 h of perfusion in the interstitial fluid of FDP-treated kidneys relative to control kidneys. After 24 h of perfusion, interstitial fluid concentrations of pyruvate were 149.1 +/- 58.4 vs. 55.6 +/- 17.9 micro M (P < 0.05) in the FDP and control group, respectively. The microdialysis probe collected the interstitial fluid directly from the cellular sites of metabolic and synthetic activity, where perfusate dilution was minimal. Consequently, the biochemi...Continue Reading

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Citations

Apr 28, 2005·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Nele Plock, Charlotte Kloft
Feb 9, 2008·Journal of Endourology·Kyle J WeldR Duane Cespedes
Feb 24, 2010·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Stefan ReuterGert Gabriëls
Sep 15, 2006·Journal of the Royal Society, Interface·Olga A BoubriakZhanfeng Cui
Sep 23, 2008·The Journal of Urology·Kyle J WeldR Duane Cespedes
Dec 20, 2008·The Journal of Urology·Kyle J WeldR Duane Cespedes
Mar 25, 2006·Clinical Transplantation·Simona C BaicuKelvin G M Brockbank
Apr 30, 2013·Transplantation Proceedings·A K KellerB Jespersen
Mar 17, 2007·Scandinavian Journal of Urology and Nephrology·Peter-Martin KrarupJorgen Froekiaer

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