Interstitial fluid osmolarity modulates the action of differential tissue surface tension in progenitor cell segregation during gastrulation

Development
S F Gabriel KrensCarl-Philipp Heisenberg

Abstract

The segregation of different cell types into distinct tissues is a fundamental process in metazoan development. Differences in cell adhesion and cortex tension are commonly thought to drive cell sorting by regulating tissue surface tension (TST). However, the role that differential TST plays in cell segregation within the developing embryo is as yet unclear. Here, we have analyzed the role of differential TST for germ layer progenitor cell segregation during zebrafish gastrulation. Contrary to previous observations that differential TST drives germ layer progenitor cell segregation in vitro, we show that germ layers display indistinguishable TST within the gastrulating embryo, arguing against differential TST driving germ layer progenitor cell segregation in vivo We further show that the osmolarity of the interstitial fluid (IF) is an important factor that influences germ layer TST in vivo, and that lower osmolarity of the IF compared with standard cell culture medium can explain why germ layers display differential TST in culture but not in vivo Finally, we show that directed migration of mesendoderm progenitors is required for germ layer progenitor cell segregation and germ layer formation.

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Citations

Apr 27, 2017·Mechanisms of Development·Carl-Philipp Heisenberg
Dec 1, 2017·Development·Natalie C Heer, Adam C Martin
Apr 7, 2020·ELife·Alexandra SchauerCarl-Philipp Heisenberg
Aug 25, 2020·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Adam C Martin
Mar 4, 2020·Development·Chii J Chan, Takashi Hiiragi
Dec 19, 2018·Nature Cell Biology·Nicoletta I PetridouCarl-Philipp Heisenberg
Feb 21, 2019·Proceedings of the National Academy of Sciences of the United States of America·Taihei FujimoriSatoshi Sawai
Mar 2, 2019·Proceedings of the National Academy of Sciences of the United States of America·Pierre RechoEdouard Hannezo
Aug 15, 2019·Annual Review of Cell and Developmental Biology·Alessandro MongeraOlivier Pourquié
Mar 14, 2021·Development·Chloé RoffayFrançois Graner
Apr 2, 2021·Biophysical Journal·Feyza Nur ArslanCarl-Philipp Heisenberg
Jul 1, 2020·Seminars in Cell & Developmental Biology·François Fagotto
Apr 24, 2021·Advanced Healthcare Materials·Ian C BergGregory H Underhill
Jul 27, 2021·Development·Chloé RoffayFrançois Graner

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Methods Mentioned

BETA
transgenic

Software Mentioned

3D
Imaris
Excel
Matlab
GraphPad Prism
CellFIT
Fiji

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