Intervention effects of five cations and their correction on hemolytic activity of tentacle extract from the jellyfish Cyanea capillata

PeerJ
Hui ZhangLi-ming Zhang

Abstract

Cations have generally been reported to prevent jellyfish venom-induced hemolysis through multiple mechanisms by spectrophotometry. Little attention has been paid to the potential interaction between cations and hemoglobin, potentially influencing the antagonistic effect of cations. Here, we explored the effects of five reported cations, La(3+), Mn(2+), Zn(2+), Cu(2+) and Fe(2+), on a hemolytic test system and the absorbance of hemoglobin, which was further used to measure their effects on the hemolysis of tentacle extract (TE) from the jellyfish Cyanea capillata. All the cations displayed significant dose-dependent inhibitory effects on TE-induced hemolysis with various dissociation equilibrium constant (Kd) values as follows: La(3+) 1.5 mM, Mn(2+) 93.2 mM, Zn(2+) 38.6 mM, Cu(2+) 71.9 μM and Fe(2+) 32.8 mM. The transparent non-selective pore blocker La(3+) did not affect the absorbance of hemoglobin, while Mn(2+) reduced it slightly. Other cations, including Zn(2+), Cu(2+) and Fe(2+), greatly decreased the absorbance with Kd values of 35.9, 77.5 and 17.6 mM, respectively. After correction, the inhibitory Kd values were 1.4 mM, 45.8 mM, 128.5 μM and 53.1 mM for La(3+), Zn(2+), Cu(2+) and Fe(2+), respectively. Mn(2+) did not inh...Continue Reading

References

Apr 18, 1972·Biochimica Et Biophysica Acta·S Razin
Dec 8, 1984·The Medical Journal of Australia·J A WilliamsonP J Fenner
Aug 5, 1998·Toxicon : Official Journal of the International Society on Toxinology·D A BloomP Alderslade
Mar 10, 2000·Toxicon : Official Journal of the International Society on Toxinology·L Edwards, D A Hessinger
Aug 31, 2000·Biochemical and Biophysical Research Communications·H NagaiT Nakajima
Feb 27, 2001·Toxicon : Official Journal of the International Society on Toxinology·J J ChungA A Yanagihara
Feb 28, 2002·Bioscience, Biotechnology, and Biochemistry·Hiroshi NagaiTerumi Nakajima
May 31, 2002·Cell and Tissue Research·Angel A YanagiharaDennis D Kunkel
Jul 13, 2004·Toxicon : Official Journal of the International Society on Toxinology·T Carrette, J Seymour
Dec 8, 2004·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Timo J NevalainenKen D Winkel
Oct 11, 2005·Bioorganic & Medicinal Chemistry Letters·Cuiping LiPengcheng Li
Feb 20, 2007·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Huahua YuPengcheng Li
Apr 13, 2007·Toxicon : Official Journal of the International Society on Toxinology·Heike HelmholzAndreas Prange
Aug 11, 2007·Toxicon : Official Journal of the International Society on Toxinology·Diane Brinkman, James Burnell
Feb 5, 2008·Toxicon : Official Journal of the International Society on Toxinology·Diane Brinkman, James Burnell
Jul 16, 2008·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·A MarinoG La Spada
Dec 17, 2008·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·A MarinoG La Spada
Mar 24, 2009·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Changkeun KangEuikyung Kim
Feb 13, 2010·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Liang XiaoLi-ming Zhang
Apr 9, 2011·Experimental and Toxicologic Pathology : Official Journal of the Gesellschaft Für Toxikologische Pathologie·Qianqian WangLiming Zhang
Nov 16, 2012·Toxicon : Official Journal of the International Society on Toxinology·Beilei WangLiming Zhang
Feb 21, 2013·Journal of Nuclear Medicine : Official Publication, Society of Nuclear Medicine·Giampaolo TomasiYiyun Huang
Apr 23, 2013·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Rongfeng LiPengcheng Li
May 22, 2013·Toxicon : Official Journal of the International Society on Toxinology·Andrew J WestonPaul F Long
Feb 11, 2014·Toxicon : Official Journal of the International Society on Toxinology·Beilei WangLiming Zhang
Mar 29, 2014·The Journal of Venomous Animals and Toxins Including Tropical Diseases·Vidal Haddad JuniorMarcos Hikari Toyama
Apr 23, 2014·Toxicon : Official Journal of the International Society on Toxinology·Changkeun KangEuikyung Kim
Jul 19, 2014·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Rossana MorabitoAngela Marino
Dec 2, 2014·The Journal of Venomous Animals and Toxins Including Tropical Diseases·Alejandro García-ArredondoJudith Sanchez-Rodriguez
Sep 12, 2015·Chembiochem : a European Journal of Chemical Biology·Lucie DupinYann Chevolot
Oct 30, 2016·Toxicon : Official Journal of the International Society on Toxinology·Yunwi HeoEuikyung Kim

❮ Previous
Next ❯

Methods Mentioned

BETA
confocal laser scanning microscopy
confocal microscopy

Software Mentioned

SPSS

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.