Intestinal and Systemic Immune Responses upon Multi-drug Resistant Pseudomonas aeruginosa Colonization of Mice Harboring a Human Gut Microbiota

Frontiers in Microbiology
Eliane von KlitzingM M Heimesaat

Abstract

The World Health Organization has rated multi-drug resistant (MDR) Pseudomonas aeruginosa as serious threat for human health. It is, however, unclear, whether intestinal MDR P. aeruginosa carriage is associated with inflammatory responses in intestinal or even systemic compartments. In the present study, we generated with respect to their microbiota "humanized" mice by human fecal microbiota transplantation of secondary abiotic mice. Following peroral challenge with a clinical P. aeruginosa isolate on two consecutive days, mice harboring a human or murine microbiota were only partially protected from stable intestinal P. aeruginosa colonization given that up to 78% of mice were P. aeruginosa-positive at day 28 post-infection (p.i.). Irrespective of the host-specificity of the microbiota, P. aeruginosa colonized mice were clinically uncompromised. However, P. aeruginosa colonization resulted in increased intestinal epithelial apoptosis that was accompanied by pronounced proliferative/regenerative cell responses. Furthermore, at day 7 p.i. increased innate immune cell populations such as macrophages and monocytes could be observed in the colon of mice harboring either a human or murine microbiota, whereas this held true at day 28...Continue Reading

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Citations

Nov 5, 2019·Journal of Leukocyte Biology·Caio Pupin RosaLeonardo Augusto de Almeida
Apr 24, 2020·Bioscience Reports·Tian-Hao LiuZhong-Shan Yang
Apr 29, 2018·Scientific Reports·Eliane von KlitzingMarkus M Heimesaat
Apr 11, 2019·European Journal of Microbiology & Immunology·Katharina MrazekMarkus M Heimesaat

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Methods Mentioned

BETA
biopsies
Assay
PCR

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