PMID: 9446830Apr 4, 1998Paper

Intestinal carbamoyl phosphate synthase I in human and rat. Expression during development shows species differences and mosaic expression in duodenum of both species

The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society
E H Van BeersJ Dekker

Abstract

The clinical importance of carbamoyl phosphate synthase I (CPSI) relates to its capacity to metabolize ammonia, because CPSI deficiencies cause lethal serum ammonia levels. Although some metabolic parameters concerning liver and intestinal CPSI have been reported, the extent to which enterocytes contribute to ammonia conversion remains unclear without a detailed description of its developmental and spatial expression patterns. Therefore, we determined the patterns of enterocytic CPSI mRNA and protein expression in human and rat intestine during embryonic and postnatal development, using in situ hybridization and immunohistochemistry. CPSI protein appeared during human embryogenesis in liver at 31-35 e. d. (embryonic days) before intestine (59 e.d.), whereas in rat CPSI detection in intestine (at 16 e.d.) preceded liver (20 e.d.). During all stages of development there was a good correlation between the expression of CPSI protein and mRNA in the intestinal epithelium. Strikingly, duodenal enterocytes in both species exhibited mosaic CPSI protein expression despite uniform CPSI mRNA expression in the epithelium and the presence of functional mitochondria in all epithelial cells. Unlike rat, CPSI in human embryos was expressed in ...Continue Reading

References

Aug 1, 1976·Archives of Biochemistry and Biophysics·H G Windmueller, A E Spaeth
Dec 1, 1992·Gastroenterology·L MaiuriS Auricchio
May 17, 1991·Biochimica Et Biophysica Acta·F BlachierW J Malaisse
Jul 1, 1990·Physiological Reviews·A J MeijerR A Chamuleau
Oct 1, 1988·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·J W Gaasbeek JanzenA F Moorman
May 1, 1989·The American Journal of Medicine·P FineD Gerstenfeld
Jul 1, 1972·The Journal of Pediatrics·J D JohnsonP Sunshine
Jan 1, 1995·Critical Reviews in Biochemistry and Molecular Biology·E H Van BeersJ Dekker
Jul 1, 1994·Gastroenterology·L MaiuriS Auricchio
Feb 1, 1994·Annals of Internal Medicine·L J WongW E O'Brien
Dec 15, 1995·The Biochemical Journal·G Wu
Dec 1, 1995·Journal of Molecular Evolution·M J van den HoffW H Lamers
Jul 26, 1996·The Journal of Biological Chemistry·C R McCudden, S G Powers-Lee
Jul 1, 1996·Histochemistry and Cell Biology·W LiouJ W Slot
Jun 15, 1995·The Biochemical Journal·E H Van BeersH A Büller
Jan 1, 1997·Gastroenterology·G R LichtensteinG T Berry

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Citations

Nov 21, 2009·American Journal of Clinical Pathology·Diana M CardonaChen Liu
Oct 20, 2005·Progress in Neuro-psychopharmacology & Biological Psychiatry·Francine GouletNazrul Islam
Jun 2, 2015·Archives of Pathology & Laboratory Medicine·Stephen LaganaHelen Remotti
Apr 22, 2016·Molecular BioSystems·Roland Nilsson, Mohit Jain
Nov 6, 2002·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Melissa VerburgJan Dekker
Mar 20, 1999·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·J SaarnioW S Sly
Jan 12, 2007·Cardiovascular Drug Reviews·Maritza J RomeroR William Caldwell
Nov 24, 1999·Current Opinion in Clinical Nutrition and Metabolic Care·Y Wakabayashi
Dec 16, 2020·Molecular Genetics and Metabolism·Matthew Nitzahn, Gerald S Lipshutz

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