Intestinal Epithelial Organoids as Tools to Study Epigenetics in Gut Health and Disease

Stem Cells International
Judith Kraiczy, Matthias Zilbauer

Abstract

The intestinal epithelium forms the inner layer of the human intestine and serves a wide range of diverse functions. Its constant exposure to a vast amount of complex microbiota highlights the critical interface that this single-cell layer forms between the host and our environment. Importantly, the well-documented contribution of environmental factors towards the functional development of the human intestinal epithelium directly implies epigenetic mechanisms in orchestrating this complex interplay. The development of intestinal epithelial organoid culture systems that can be generated from human tissue provides researchers with unpresented opportunities to study functional aspects of human intestinal epithelial pathophysiology. In this brief review, we summarise existing evidence for the role of epigenetics in regulating intestinal epithelial cell function and highlight the great potential for human gut organoids as translational research tools to investigate these mechanisms in vitro.

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Citations

Jul 18, 2020·International Journal of Molecular Sciences·Elena NaumovskaDorota Kurek
Jul 1, 2020·Tissue Engineering and Regenerative Medicine·Harikrishna Reddy RallabandiBo Ram Lee
Jan 10, 2020·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Alexis Pierre ArnaudGaëlle Boudry
Sep 27, 2019·Food Research International·Lívia Beatriz Aparecida Ribeiro SilvaThomas Prates Ong
Dec 21, 2020·Journal of Crohn's & Colitis·Martina PolettiTamas Korcsmaros
Feb 13, 2021·Frontiers in Cell and Developmental Biology·Jenny OstropMenno J Oudhoff
Mar 12, 2021·Veterinary Research·Soumya K KarHenri Woelders
Feb 18, 2021·Journal of Crohn's & Colitis·Sho WatanabeKiichiro Tsuchiya

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Methods Mentioned

BETA
biopsies
IEC
biopsy
methylation profiling

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