Intestinal microbiome as a risk factor for urinary tract infections in children

European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology
Niko PaalanneTerhi Tapiainen

Abstract

As urinary tract infection (UTI) pathogens originate from the gut, we hypothesized that the gut environment reflected by intestinal microbiome influences the risk of UTI. Our prospective case-control study compared the intestinal microbiomes of 37 children with a febrile UTI with those of 69 healthy children. We sequenced the regions of the bacterial 16S rRNA gene and used the LefSe algorithm to calculate the size of the linear discriminant analysis (LDA) effect. We measured fecal lactoferrin and iron concentrations and quantitative PCR for Escherichia coli. At the phylum level, there were no significant differences. At the genus level, Enterobacter was more abundant in UTI patients with an LDA score > 3 (log 10), while Peptostreptococcaceae were more abundant in healthy subjects with an LDA score > 3 (log 10). In total, 20 OTUs with significantly different abundances were observed. Previous use of antimicrobials did not associate with intestinal microbiome. The relative abundance of E. coli was 1.9% in UTI patients and 0.5% in controls (95% CI of the difference-0.8 to 3.6%). The mean concentration of E.coli in quantitative PCR was 0.14 ng/μl in the patients and 0.08 ng/μl in the controls (95% CI of the difference-0.04 to 0.16)...Continue Reading

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Citations

Dec 5, 2019·Nature Communications·Matthew MagruderJohn Richard Lee
Apr 8, 2021·Pediatric Research·Sofia AinonenTerhi Tapiainen
May 12, 2020·Urology·Tara Curley, Catherine S Forster
Jun 5, 2021·Frontiers in Cellular and Infection Microbiology·Virginia Perez-CarrascoJose A Garcia-Salcedo
Aug 10, 2021·Frontiers in Cellular and Infection Microbiology·Jonathan Josephs-SpauldingChristoph Kaleta

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Datasets Mentioned

BETA
SRP125791

Methods Mentioned

BETA
urine collection
PCR
electrophoresis
chip
ELISA

Software Mentioned

LCS480
SPSS
FastTree2
LightCycler 480
Usearch
Ion
LEfSe
Abs Quant
RDP
QIIME split _ libraries . py

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