Intestinal Microbiota and Immune Modulation in Zebrafish by Fucoidan From Okinawa Mozuku (Cladosiphon okamuranus )

Frontiers in Nutrition
Wakako Ikeda-OhtsuboSylvia Brugman

Abstract

Fucoidan represents fucose-rich sulfated polysaccharides derived from brown seaweeds, which exerts various biological activities applicable for functional foods and therapeutic agents. The objective of the present study was to investigate in vivo effects of fucoidan extracted from Okinawa mozuku (Cladosiphon okamuranus), common edible seaweed in Japan, on immune responses and microbiota composition in zebrafish. We treated larvae and adult zebrafish with Okinawa mozuku (OM) fucoidan by immersion (100 and 500 μg/mL, 3 days) and by feeding (3 weeks), respectively. The effect of OM fucoidan on immune responses in zebrafish larvae was evaluated by live imaging of neutrophils and macrophages as well as quantitative polymerase chain reaction of pro- and anti-inflammatory cytokine genes. Whole microbiota of zebrafish larvae and intestinal microbiota of adult zebrafish treated with OM fucoidan were analyzed by Illumina MiSeq pair-end sequencing of the V3-V4 region of 16S rRNA genes. Fucoidan treatment only slightly affected the composition of the larvae microbiota and the number of neutrophils and macrophages, while pro- and anti-inflammatory cytokine gene expression levels were upregulated in the larvae treated with 500 μg/mL OM fucoi...Continue Reading

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Citations

Jun 2, 2021·Critical Reviews in Food Science and Nutrition·Fang-Jun LinRen-You Gan
Jun 3, 2021·Animals : an Open Access Journal From MDPI·Abdel-Wahab A Abdel-WarithMahmoud A O Dawood
Jul 3, 2021·Marine Drugs·Emer ShannonMaria Hayes
Aug 27, 2021·Marine Drugs·Jin-Young Yang, Sun Young Lim

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Datasets Mentioned

BETA
PRJNA597701

Methods Mentioned

BETA
transgenic
Fluorescence
dissection
Illumina sequencing
PCR
amplicon sequencing

Software Mentioned

Excel®
DESeq2
Linear discriminant analysis effect size ( LEfSe )
Microbiomeanalyst©
CLC Bio Genomics Workbench
MICCA
LEfSe
EZbioCloud
R
ImageJ®

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