Intestinal microbiota differentially affect brush border enzyme activity and gene expression in the neonatal gnotobiotic pig

Journal of Animal Physiology and Animal Nutrition
B P Willing, A G Van Kessel

Abstract

To study microbial influence on intestinal development pertaining to nutrient digestion, two separate gnotobiotic experiments were performed, each with 16 piglets allocated to four treatment groups: germfree (GF), monoassociation with Escherichia coli, monoassociation with Lactobacillus fermentum or conventionalization with faecal bacteria (CV). Enzyme activity and gene expression of lactase phlorizin hydrolase (LPH) and aminopeptidase N (APN) were measured in isolated enterocytes, harvested on day 14, using specific substrates and quantitative PCR respectively. Enterocytes of CV pigs had reduced APN activity, but had increased gene expression relative to GF, making the specific activity:mRNA (A:G) ratio dramatically lower (p < 0.05). Similarly, LPH A:G ratio was significantly reduced (p < 0.05) in enterocytes of CV pigs as compared with GF. The results of co-incubation of L. fermentum, E. coli and faecal bacteria with APN indicate a direct relationship between enzyme inactivation and specific A:G ratio in enterocytes. We conclude that enterocyte up-regulation of APN expression occurs as either a direct response to microbial colonization or as a feedback mechanism in response to reduced enzyme activity through microbial degrada...Continue Reading

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Citations

Aug 2, 2012·MBio·Lindsay M Gielda, Victor J DiRita
Mar 6, 2010·American Journal of Physiology. Gastrointestinal and Liver Physiology·Robert S ChapkinEdward R Dougherty
May 16, 2012·Advances in Nutrition·Sharon M DonovanRobert S Chapkin
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Jun 5, 2021·BMC Microbiology·Jinhee KimRonaldo P Ferraris
Nov 11, 2021·Development·Lihua Ye, John F Rawls

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