Intestinal microbiota succession and immunomodulatory consequences after introduction of Lactobacillus reuteri I5007 in neonatal piglets

PloS One
Chengli HouShiyan Qiao

Abstract

Seventy-two, suckling piglets, obtained from 9 litters standardized to 8 piglets, were assigned to 1 of 3 treatments (n = 24) to compare short-term, early administration with intermittent, longer-term administration of Lactobacillus reuteri I5007. The treatments were a control (given a placebo of 0.1% peptone water from day 1 to 5) or treatments in which 1.7 × 1010 CFU L. reuteri was administrated either daily for 4 days starting on day 1 or every 4th day from day 1 to 17. Five piglets per treatment were killed at 3 time points (day 7, 14 and 21). Denaturing Gradient Electrophoresis of ileal digesta revealed an increase in the presence of L. reuteri I5007 and Clostridium lentocellum (on day 14 and 21) in the every 4th-day treatment and Actinobacillus porcinus (on day 7 and 14) in both L. reuteri treatments, while reducing the abundance of E. coli on day 21 in the every 4th-day treatment. Real-time qPCR of ileal digesta showed an increase in Bifidobacterium spp. on day 14 for both L. reuteri I5007 treatments. An increase in the concentration of lactic acid and a lower pH was observed in the first 4-day treatment on day 7 and the every 4th day treatment on day 14. The relative abundance of mRNA for TGF-β was increased while that ...Continue Reading

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Citations

May 21, 2017·Applied and Environmental Microbiology·Ursula M McCormackPeadar G Lawlor
Oct 11, 2017·Journal of Animal Physiology and Animal Nutrition·B MorissetteM Lessard
Jul 1, 2020·Frontiers in Microbiology·Clare H GaukrogerIlias Kyriazakis

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Datasets Mentioned

BETA
HF952859-HF952864

Methods Mentioned

BETA
electrophoresis
PCR

Software Mentioned

Statistical Analysis Systems Statistical
BLAST
Quantity One Basic

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