PMID: 43948Jan 1, 1979

Intestinal microflora and bile acids. In vitro cholic acid transformation by mixed fecal culture of rats

Microbiology and Immunology
M MorotomiM Mutai

Abstract

In vitro cholic acid (CA) transformation by mixed fecal culture was investigated. Concentrations of glucose, peptone, and yeast extract in the medium and the initial pH of the medium markedly affected the CA transformation. Yeast extract enhanced the transformation, whereas high concentrations of glucose and peptone inhibited it. When the initial pH of the medium was below 6.5, CA was converted to 7-keto-deoxycholic acid (7KD), and formation of deoxycholic acid (DC) was not observed. In contrast, with an initial pH of 7.0, about 60% of the CA was converted to 7KD after 3 days of incubation, and then DC gradually formed after 4 days of incubation, following the disappearance of 7KD. The formation of DC in the cultured samples was paralleled in each case by disappearance of 7KD. In pure culture systems, Escherichia coli and some strains of Bacteroides formed 7KD from CA. No DC formation was observed in pure cultures of any of the strains examined.

References

Sep 1, 1978·Steroids·I A MacdonaldC E Meier
Oct 1, 1977·Applied and Environmental Microbiology·M R SadzikowskiT D Wilkins
Oct 1, 1978·The American Journal of Clinical Nutrition·E J Stellwag, P B Hylemon
Aug 1, 1975·Journal of Medical Microbiology·P GoddardP Barnes
May 5, 1970·Biochimica Et Biophysica Acta·V Aries, M J Hill
Dec 1, 1974·The American Journal of Clinical Nutrition·M J Hill
Jan 1, 1968·Acta Pathologica Et Microbiologica Scandinavica·T Midtvedt, A Norman
Feb 1, 1966·The Journal of Experimental Medicine·B E GustafssonA Norman
Feb 1, 1968·Gut·M J Hill, B S Drasar

Citations

Sep 1, 1991·The British Journal of Nutrition·E Leng-Peschlow
Jan 1, 1985·Critical Reviews in Toxicology·I R RowlandA Wise

Related Concepts

7-ketodeoxycholic acid, (3alpha,12alpha)-isomer
Cholic Acids
Deoxycholic Acid, Sodium Salt, 12beta-Isomer
Feces
Hydrogen-Ion Concentration

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