Intestinal neuro-epithelial interactions modulate neuronal chemokines production

Biochemical and Biophysical Research Communications
Emmanuelle TixierMichel Neunlist

Abstract

Human enteric neurons have recently been shown to produce chemokines during intestinal inflammation. However, whether (1) neuro-epithelial interactions modulate neuronal chemokines production and (2) neurons can induce the chemotaxis of immune cells remain unknown. Neuro-epithelial interactions were studied using a coculture model composed of human neurons (NT2-N) and intestinal epithelial cells (Caco-2). IL-8 or MIP-1beta expression was analyzed by quantitative-PCR, ELISA or immunohistochemistry. Neuronally induced chemotaxis was studied using a coculture model composed of NT2-N and human peripheral blood mononuclear cells (PBMC). Following Caco-2 inflammation with IFNgamma/TNFalpha, neuronal IL-8 and MIP-1beta mRNA expression was significantly increased compared to control. This increase was significantly reduced by IL-1 receptor antagonist. IL-1beta-pretreated NT2-N induced the chemotaxis of PBMC, which was significantly reduced by anti-IL-8, but not by anti-MIP-1beta neutralizing antibody. Our results demonstrate that, under inflammatory conditions, neuro-epithelial interactions can modulate neuronal chemokines production through IL-1beta-dependent pathways. Furthermore, neuronal IL-1beta-induced chemotactic properties coul...Continue Reading

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Citations

Sep 26, 2008·Inflammatory Bowel Diseases·Giovanni BarbaraRoberto Corinaldesi
Aug 20, 2010·Inflammatory Bowel Diseases·Matthias A EngelMarkus F Neurath
Nov 29, 2012·Investigative Ophthalmology & Visual Science·Haochuan LiPeter W Chen
Sep 18, 2009·Digestive Diseases and Sciences·Francisco Javier Guzmán-de la GarzaNancy Esthela Fernández-Garza
Aug 16, 2011·Pharmacology & Therapeutics·Pim J KoelinkAletta D Kraneveld
Dec 16, 2006·Autonomic Neuroscience : Basic & Clinical·Luc Van NassauwJean-Pierre Timmermans
Jun 27, 2012·Annals of the New York Academy of Sciences·Maren AmashehJörg-Dieter Schulzke
Mar 24, 2009·Biochemical and Biophysical Research Communications·Raphaël MoriezMichel Neunlist
Apr 18, 2008·European Journal of Pharmacology·Aletta D KraneveldJohan Garssen
Jul 22, 2008·Journal of Neuroscience Methods·Dmitry IvanovValery I Shestopalov
Aug 16, 2011·Biochemical and Biophysical Research Communications· AshutoshAnuja Ghorpade
Aug 5, 2008·Current Opinion in Pharmacology·Shomron Ben-Horin, Yehuda Chowers
Nov 21, 2012·Nature Reviews. Gastroenterology & Hepatology·Michel NeunlistMalvyne Rolli-Derkinderen
May 11, 2010·Immunological Investigations·Paul Forsythe, John Bienenstock
Aug 10, 2020·Current Opinion in Supportive and Palliative Care·Rachel M McQuadeKulmira Nurgali
Dec 17, 2014·American Journal of Physiology. Gastrointestinal and Liver Physiology·Vanesa StojanovskaKulmira Nurgali
Feb 23, 2018·Frontiers in Immunology·Mohammad KhalilMatthias Agop Engel
May 18, 2020·Current Drug Targets·Rhian StavelyKulmira Nurgali

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