PMID: 9630621Jun 19, 1998Paper

Intestinal NF-kappaB is activated, mainly as p50 homodimers, by platelet-activating factor

Biochimica Et Biophysica Acta
Isabelle G De PlaenW Hsueh

Abstract

NF-kappaB, a transcription factor, upregulates gene transcription of many inflammatory mediators. Here, we examined the activity of NF-kappaB in the rat small intestine, and how it may be affected by platelet-activating factor (PAF), an important mediator for intestinal injury and inflammation. Ileal nuclear extracts from sham-operated and PAF (1.5 microg/kg)-injected rats were prepared for the assessment of NF-kappaB DNA-binding activity, and the identification of NF-kappaB subunits. The experiment was also performed on neutrophil-depleted rats to examine whether the PAF effect is neutrophil-dependent. Cellular NF-kappaB was localized by immunohistochemistry. We found that: (a) NF-kappaB is constitutively active in rat small intestine; (b) PAF at a dose below that causing shock and bowel necrosis enhances DNA-binding activity of NF-kappaB within 30 min after injection; activated NF-kappaB contains predominantly p50 subunits; (c) immunohistochemistry showed that PAF induced translocation of p50 into the nucleus of cells of the lamina propria, as well as of the epithelium; and (d) the effect of PAF is abrogated by neutrophil depletion, suggesting a role of neutrophils in NF-kappaB activation. Our study suggests that NF-kappaB is...Continue Reading

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Citations

Sep 25, 1999·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·T SakaguchiT Okamoto
May 23, 2009·American Journal of Physiology. Gastrointestinal and Liver Physiology·Shirley X L LiuIsabelle G De Plaen
Jan 7, 2014·Pathophysiology : the Official Journal of the International Society for Pathophysiology·Catherine J Hunter, Isabelle G De Plaen
Feb 19, 2013·Clinics in Perinatology·Isabelle G De Plaen
Mar 14, 2009·Biochemical Pharmacology·Denise M MarottaJoão B Calixto
Jul 19, 2005·British Journal of Pharmacology·Elizabeth S FernandesJoão B Calixto
Sep 10, 2005·Journal of Pediatric Surgery·Alaa CheikhelardSabine Sarnacki
Aug 24, 1999·The Journal of Surgical Research·T SakaguchiS Baba
Jul 2, 2004·American Journal of Physiology. Cell Physiology·Ryoko MinekawaYuji Murata
Aug 21, 2004·American Journal of Physiology. Gastrointestinal and Liver Physiology·Xin-Bing HanIsabelle G De Plaen
Nov 9, 2002·Pediatric and Developmental Pathology : the Official Journal of the Society for Pediatric Pathology and the Paediatric Pathology Society·Wei HsuehF Gonzalez-Crussi
Feb 7, 2001·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·X W QuW Hsueh
Oct 12, 1999·The American Journal of Physiology·N MerendinoM F Kagnoff
Nov 30, 2000·American Journal of Physiology. Gastrointestinal and Liver Physiology·M S InanC Giardina
May 23, 2002·American Journal of Physiology. Gastrointestinal and Liver Physiology·Heitham T HassounBruce C Kone
Dec 30, 2018·The American Journal of Pathology·Elizabeth ManagliaIsabelle G De Plaen
Aug 5, 2000·Biochemical and Biophysical Research Communications·Y H ChenX D Tan

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