PMID: 2106270Feb 1, 1990Paper

Intestinal ornithine decarboxylase: half-life and regulation by putrescine

The American Journal of Physiology
K IwamiL R Johnson

Abstract

Ornithine decarboxylase (ODC) is the primary rate-limiting enzyme for polyamine synthesis. ODC levels are increased in most tissues, including the intestinal mucosa, by growth-promoting agents. This enzyme has a brief half-life of from 5 to 30 min in mammalian tissues and is regulated by its product; putrescine. The current study examines the turnover and regulation of ODC in the mucosa of the small intestine. With the use of scraped intestinal mucosa from cycloheximide-treated rats, the time course of the decline in ODC activity yielded a half-life of approximately 22 min. Labeling enzyme protein with [3H]difluoromethylornithine (DFMO) resulted in a nearly identical estimation of half-life. ODC activity of mucosa from isolated gut segments stimulated by luminal glycine (0.1-0.4 M) was enhanced 60-100% by 10 mM putrescine administered luminally. Putrescine alone had no effect on ODC. In contrast, 10(-7) M putrescine prevented 80% of the ODC activity stimulated by asparagine in IEC-6 cells (a rat intestinal crypt cell line). The half-life of ODC in unstimulated IEC-6 cells was 20 min and increased to 35 min in cells exposed to 10 mM asparagine. These data demonstrate that ODC of nonproliferating villous cells is regulated differ...Continue Reading

Citations

Jun 9, 2006·American Journal of Physiology. Endocrinology and Metabolism·David L WilliamsonLeonard S Jefferson
Dec 21, 2000·American Journal of Physiology. Gastrointestinal and Liver Physiology·Q YuanL R Johnson
Jun 3, 2021·Medical Sciences : Open Access Journal·Shima Nakanishi, John L Cleveland
Apr 10, 2020·Accounts of Chemical Research·Kyle W RoskampRachel W Martin
Aug 28, 2018·Chemical Reviews·Saba ParvezYimon Aye
Oct 30, 2021·PLoS Neglected Tropical Diseases·Adriana TemporãoLuisa M Figueiredo

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