Intestinal permeability of chlorpyrifos using the single-pass intestinal perfusion method in the rat

Toxicology
Thomas J Cook, Smriti S Shenoy

Abstract

The intestinal transport of chlorpyrifos (CPF), an organothiophosphate pesticide, was investigated using the single-pass intestinal perfusion (SPIP) technique in male, Sprague-Dawley rats. SPIP was performed in each isolated region of the small intestine (i.e. duodenum, jejunum and ileum) with three concentrations of CPF (0.1, 2.0 and 10 microM) at a flow rate of 0.25 ml/min. Preliminary binding and stability studies were conducted to ensure that the loss of CPF in the SPIP study can be attributed to intestinal absorption. The effective permeability (P(eff)) of CPF was determined for each segment and concentration. CPF exhibits a high intestinal permeability over the length of the small intestine indicative of compounds that are well absorbed. Decreases in permeability values at the highest CPF concentration studied in the duodenum and ileum suggest a saturable transport process. Based on these results, passive, transcellular diffusion dominates the intestinal transport mechanism of CPF, with a saturable transport process evident in the duodenum and ileum. The P(eff) of CPF is in the range of drugs with high intestinal permeability and high fraction of dose absorbed indicating that CPF readily crosses the intestine. The depende...Continue Reading

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Citations

Jun 23, 2011·Indian Journal of Pharmaceutical Sciences·N NagareMeena Chintamaneni
May 30, 2013·Indian Journal of Pharmaceutical Sciences·J R Patel, Kalyani H Barve
Sep 17, 2010·Journal of Pharmaceutical Sciences·Hong LiJumah Masoud
Jan 23, 2013·Journal of Pharmacological and Toxicological Methods·André Bersani DezaniCristina Helena Dos Reis Serra
Oct 6, 2012·Toxicology Letters·Claudia LeoniEmanuela Testai
Jun 7, 2013·The Journal of Pharmacy and Pharmacology·Michael P WhiteManoj Maniar
Jul 17, 2012·International Journal of Pharmaceutics·Jianjun ZhangShuai Qian
Feb 27, 2013·The Korean Journal of Physiology & Pharmacology : Official Journal of the Korean Physiological Society and the Korean Society of Pharmacology·Soung Baek JangJaehwi Lee
Jul 16, 2014·PloS One·Claire Joly CondetteJérôme Gay-Quéheillard
Nov 10, 2016·International Journal of Environmental Research and Public Health·Julie ReygnerHafida Khorsi-Cauet
Nov 12, 2015·Journal of Exposure Science & Environmental Epidemiology·Thomas J WeberCharles Timchalk
May 28, 2010·Toxicology and Industrial Health·Sarojni Tripathi, Ajai Kumar Srivastav
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Jul 17, 2010·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Yi-Tong LiuGuang-Ji Wang
Jan 1, 2013·Journal of Pharmaceutics·Bharat Bhushan Subudhi, Surjyanarayan Mandal
Aug 12, 2020·Medicinal Research Reviews·Lipeng WuBaiyi Lu
Apr 11, 2021·European Journal of Nutrition·Narimane DjekkounHafida Khorsi-Cauet

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