Intestinal Shiga toxin-producing Escherichia coli bacteria mitigate bovine leukemia virus infection in experimentally infected sheep.

Infection and Immunity
Witold A FerensCarolyn J Hovde

Abstract

Ruminants often carry gastrointestinal Shiga toxin (Stx)-producing Escherichia coli (STEC). Stxs belong to a large family of ribosome-inactivating proteins (RIPs), found in many plants and some bacteria. Plant RIPs, secreted into extracellular spaces, limit the spread of viruses through plant tissues by penetrating and killing virally infected cells. Previously, we showed Stx activity against bovine leukemia virus (BLV)-infected cells in vitro and hypothesized that STEC bacteria have antiviral activity in ruminant hosts. Here, we investigated the impact of STEC on the initial phases of BLV infection in sheep. Sheep were treated with biweekly oral doses of E. coli O157:H7 (an STEC) or an isogenic stx mutant strain. A different group of sheep were similarly treated with five naturally occurring ovine STEC isolates or stx-negative E. coli. Intestinal STEC bacteria were enumerated and identified by standard fecal culture and DNA hybridization. Oral STEC treatment did not always result in carriage of STEC, although many animals consistently presented with >10(4) CFU/g feces. BLV viremia was assessed by spontaneous lymphocyte proliferation (SLP) in cultures of blood mononuclear cells and by syncytium formation in cocultures of the sa...Continue Reading

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Feb 24, 2004·Infection and Immunity·Witold A FerensCarolyn J Hovde

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Citations

Dec 2, 2010·Foodborne Pathogens and Disease·Witold A Ferens, Carolyn J Hovde
Nov 7, 2006·Applied and Environmental Microbiology·Nancy A CornickVijay Sharma
Apr 10, 2012·Bioscience, Biotechnology, and Biochemistry·Ali Jahanian-NajafabadiLorenz M Mayr
Jan 2, 2007·Virus Research·Witold A FerensCarolyn J Hovde
Aug 22, 2008·Journal of Veterinary Science·Jang W Yoon, Carolyn J Hovde

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