Intestinal Transcriptome Analysis Highlights Key Differentially Expressed Genes Involved in Nutrient Metabolism and Digestion in Yellowtail Kingfish (Seriola lalandi) Fed Terrestrial Animal and Plant Proteins.

Genes
Chinh Thi My DamAbigail Elizur

Abstract

This study investigated the effects of dietary terrestrial animal and plant proteins on the intestinal transcriptomes of yellowtail kingfish (YTK), Seriola lalandi, an ecologically and economically important marine species in Australia. Five diets containing fish meal (FM), poultry by-product meal (PBM), blood meal (BLM), faba bean meal (FBM) and corn gluten meal (CGM) were formulated and fed over a period of 4 weeks. The Illumina RNA-sequencing (RNA-Seq) results identified a suite of differentially expressed genes involved in nutrient metabolism and protein digestion pathways, reinforced by quantitative polymerase chain reaction (qPCR) results. These findings provide molecular support to the notion that PBM and FBM are useful raw materials in commercial diets for YTK. Using the same evidence, we have demonstrated that BLM and CGM may be less useful and their incorporation into commercial aquafeeds for this species should be done cautiously. The differentially expressed genes showed a subtle difference and high correlation with apparent nutrient digestibility of raw materials. Further, our results indicate that transcriptome profiling provides a useful tool to evaluate alternative protein sources for use in aquaculture feeds.

References

Sep 11, 2007·Biochimie·Klaudia BrixSilvia Jordans
Sep 16, 2008·Fish Physiology and Biochemistry·P EnesA Oliva-Teles
May 31, 2014·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Marta BouIsabel Navarro
Apr 29, 2015·Comparative Biochemistry and Physiology. Part D, Genomics & Proteomics·Christian De SantisDouglas R Tocher
Nov 3, 2015·International Journal of Biological Macromolecules·Bo-Guang Sun, Heng Chi
Mar 21, 2016·Developmental and Comparative Immunology·Samuel A M MartinElżbieta Król
May 10, 2017·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Stefan de GelderPeter H M Klaren
Oct 18, 2018·DNA Research : an International Journal for Rapid Publication of Reports on Genes and Genomes·Motoshige YasuikeAtushi Fujiwara

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Datasets Mentioned

BETA
SRR11196438
PRJNA609208

Methods Mentioned

BETA
Assay
PCR
RNA-Seq

Software Mentioned

Blastx
CLC genomics workbench
GSEA
Blast
DEseq2
SPSS
Blast2Go Set Enrichment Analysis ( GSEA )

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