Intestinal transport of the lactokinin Ala-Leu-Pro-Met-His-Ile-Arg through a Caco-2 Bbe monolayer

Journal of Peptide Science : an Official Publication of the European Peptide Society
Vanessa VermeirssenW Verstraete

Abstract

ACE inhibitory peptides are biologically active peptides that play a role in blood pressure regulation. When derived from food proteins during food processing or gastrointestinal digestion, these peptides could function as efficient agents in treating and preventing hypertension. However, in order to exert an antihypertensive effect by inhibition of the ACE enzyme, they have to reach the bloodstream intact. The aim of this research was to assess if the known ACE inhibitory peptide Ala-Leu-Pro-Met-His-Ile-Arg, derived from a tryptic digest of beta-lactoglobulin, could be absorbed through a Caco-2 Bbe cell monolayer in an Ussing chamber and reach the serosal side undegraded. Samples of the mucosal compartment showed high ACE inhibitory activity. No or only little ACE inhibitory activity was detected in the serosal compartment. However, when the serosal sample was concentrated three-fold, a substantial ACE inhibitory activity was registered. Concomitantly, HPLC and MS clearly showed the presence of Ala-Leu-Pro-Met-His-Ile-Arg in the mucosal compartment, whereas in the serosal compartment only MS was able to detect the heptapeptide. In conclusion. under the observed experimental conditions, the ACE inhibitory peptide Ala-Leu-Pro-Me...Continue Reading

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Citations

Oct 8, 2004·The British Journal of Nutrition·Vanessa VermeirssenWilly Verstraete
Aug 12, 2008·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Le Hoang LamHiroyuki Ukeda
Oct 16, 2004·Journal of Dairy Science·D J WalshR J Fitzgerald
Jan 29, 2010·Journal of Dairy Science·A R MadureiraF X Malcata
Apr 17, 2010·Molecular Nutrition & Food Research·Daniela RegazzoRachel Boutrou
Apr 26, 2016·Nutrition Research Reviews·Katy HornerLorraine Brennan
Feb 28, 2014·The Journal of Nutrition·Hannah D HolscherKelly A Tappenden
Nov 11, 2003·The Journal of Nutrition·Aurélien TrompettePascale Plaisancié
Dec 29, 2018·Journal of Dairy Science·Carlotta GirominiAntonella Baldi
Jul 18, 2017·Journal of the Science of Food and Agriculture·Yuxing GuoDaodong Pan
Feb 22, 2007·The Journal of Nutrition·Tiina Jauhiainen, Riitta Korpela
Feb 3, 2021·Comprehensive Reviews in Food Science and Food Safety·Lu XuePangzhen Zhang
Mar 31, 2004·The Journal of Nutrition·Richard J FitzGeraldDaniel J Walsh

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