Intestinal-type fibroblasts selectively influence proliferation rate and peptide synthesis in the murine entero-endocrine cell line STC-1

Differentiation; Research in Biological Diversity
C RatineauC Roche

Abstract

The intestinal epithelium consists of enterocytes, endocrine cells, goblet cells and Paneth cells, which differentiate from pluripotent stem cells located at the crypt bases. The role of the epithelial-mesenchymal inter-actions has been well documented for the differentiation of enterocytes, but the mechanisms that control endocrine cell differentiation are poorly understood. We have cultured the intestinal endocrine cell line STC-1, which synthesizes most of the intestinal peptide hormones, in media conditioned by several subepithelial fibroblast cell lines from three distinct sites of intestine. The fibroblast Swiss 3T3 cell line was used as a non-intestinal control. Our results show that culture media from intestinal fibroblasts inhibit the proliferation rate of STC-1 cells, while those from Swiss 3T3 fibroblasts do not. As regards peptide hormone gene expression, Swiss 3T3-conditioned media have no effect, whereas media from intestinal fibroblasts variably affect cholecystokinin, glucagon, secretin and somatostatin mRNA levels. In particular, clonal subepithelial myofibroblasts do not exert the same effects as mixed subepithelial fibroblasts from homologous intestinal segment. Taken together, these results suggest that cult...Continue Reading

References

Jun 1, 1990·Differentiation; Research in Biological Diversity·A Andrew, B B Rawdon
Jul 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·I CornilR S Kerbel
Mar 1, 1980·Analytical Biochemistry·C Labarca, K Paigen
Mar 1, 1996·Virchows Archiv : an International Journal of Pathology·S L AsaD J Drucker
Apr 9, 1996·Regulatory Peptides·J D TuckerP L Brubaker
Aug 1, 1996·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·M JackerottL I Larsson
Oct 1, 1996·The Journal of Endocrinology·C RatineauJ A Chayvialle

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Citations

Oct 7, 2011·In Vitro Cellular & Developmental Biology. Animal·Tetsuji YoshikawaAkeo Hagiwara
Apr 20, 2000·European Journal of Pharmacology·A MuraiG J Dockray
Apr 28, 2001·Molecular and Cellular Endocrinology·C RatineauC Roche
May 19, 2006·American Journal of Physiology. Cell Physiology·Monica C ChenEnrique Rozengurt
Jun 5, 1998·The American Journal of Physiology·M PlaterotiM Kedinger

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