Intimin from enteropathogenic Escherichia coli restores murine virulence to a Citrobacter rodentium eaeA mutant: induction of an immunoglobulin A response to intimin and EspB.

Infection and Immunity
G FrankelG Dougan

Abstract

The formation of attaching and effacing (A/E) lesions is central to the pathogenesis of enteropathogenic Escherichia coli (EPEC)-mediated disease in humans and Citrobacter rodentium (formerly C. freundii biotype 4280)-mediated transmissible colonic hyperplasia in mice. Closely related outer membrane proteins, known as intimins, are required for formation of the A/E lesion by both EPEC (Int(EPEC)) and C. rodentium (Int(CR)). A secreted protein, EspB (formally EaeB), is also necessary for A/E-lesion formation. Here we report that expression of a cloned Int(EPEC), encoded by plasmid pCVD438, restores murine virulence to an intimin-deficient mutant of C. rodentium DBS255. Replacement of Cys937 with Ala abolished the ability of the cloned EPEC intimin to complement the deletion mutation in DBS255. Ultrastructural examination of tissues from wild-type C. rodentium and DBS255(pCVD438)-infected mice revealed multiple A/E lesion on infected cells and loss of contact between enterocytes and basement membrane. Histological investigation showed that although both wild-type C. rodentium and DBS255(pCVD438) colonized the descending colon and induced colonic hyperplasia in orally infected 21-day-old mice, the latter strain adhered to epitheli...Continue Reading

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Citations

Feb 2, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Cameron P SimmonsThomas T MacDonald
Mar 15, 2003·Journal of Food Protection·Jeffrey J VarcoeLinda Brady
Jan 25, 2005·Infection and Immunity·Suzana D SavkovicGail Hecht
Dec 14, 2004·European Journal of Immunology·Tania K UrenRichard A Strugnell
Apr 9, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Mitsutoshi IimuraMartin F Kagnoff
Nov 29, 2005·Cellular Microbiology·Rosanna MundySiouxsie Wiles
May 6, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Sara M DannLars Eckmann
Aug 6, 2013·Journal of Bacteriology·Shahista NisaMichael S Donnenberg
Apr 27, 1999·Molecular Microbiology·E L HartlandG Frankel
Jun 26, 2002·Journal of Pediatric Gastroenterology and Nutrition·Alan D Phillips
Jun 6, 2003·Pediatric Allergy and Immunology : Official Publication of the European Society of Pediatric Allergy and Immunology·Cristiane Barros CarbonareMagda Maria Sales Carneiro-Sampaio
May 23, 2003·Infection and Immunity·Bruce A VallanceB Brett Finlay
Dec 18, 2013·Immunology·Andrew J McDermott, Gary B Huffnagle
Oct 8, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Chris K F LiThomas T MacDonald
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Oct 14, 2000·Annual Review of Cell and Developmental Biology·D L GoosneyB B Finlay

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