Intra- and interlaboratory study of a method for testing the antifungal susceptibilities of dermatophytes

Journal of Clinical Microbiology
Mahmoud A GhannoumDavid W Warnock

Abstract

The National Committee for Clinical Laboratory Standards (NCCLS) M38-A standard for the susceptibility testing of conidium-forming filamentous fungi does not explicitly address the testing of dermatophytes. This multicenter study, involving six laboratories, investigated the MIC reproducibility of seven antifungal agents tested against 25 dermatophyte isolates (5 blinded pairs of five dermatophyte species per site for a total of 300 tests), using the method of dermatophyte testing developed at the Center for Medical Mycology, Cleveland, Ohio. The dermatophytes tested included Trichophyton rubrum, Trichophyton mentagrophytes, Trichophyton tonsurans, Epidermophyton floccosum, and Microsporum canis. Seven antifungals with activity against dermatophytes were tested, including ciclopirox, fluconazole, griseofulvin, itraconazole, posaconazole, terbinafine, and voriconazole. Interlaboratory MICs for all isolates were in 92 to 100% agreement at a visual endpoint reading of 50% inhibition as compared to the growth control and 88 to 99% agreement at a visual endpoint reading of 80% inhibition as compared to the growth control. Intralaboratory MICs between blinded pairs were in 97% agreement at a visual endpoint reading of 50% inhibition ...Continue Reading

References

Sep 1, 1990·Antimicrobial Agents and Chemotherapy·M A PfallerF C Odds
Dec 25, 2002·Antimicrobial Agents and Chemotherapy·Pranab K MukherjeeMahmoud A Ghannoum
Oct 25, 2003·Antimicrobial Agents and Chemotherapy·Colin S OsborneNeil S Ryder

❮ Previous
Next ❯

Citations

Nov 9, 2012·The Journal of Antimicrobial Chemotherapy·Ludmila de Matos BaltazarPatrícia Silva Cisalpino
Jul 3, 2013·Antimicrobial Agents and Chemotherapy·M GhannoumB Hardas
Mar 21, 2007·Antimicrobial Agents and Chemotherapy·Zhongsheng TongSharon C A Chen
Apr 9, 2005·Journal of Clinical Microbiology·D A Santos, J S Hamdan
May 15, 2008·Mycopathologia·Nilce M Martinez-RossiAntonio Rossi
Aug 6, 2010·International Journal of Antimicrobial Agents·Michael ArabatzisAliki Voyatzi
Oct 31, 2006·Mycological Research·Maria Elisabete da Silva BarrosJúnia Soares Hamdan
Aug 24, 2007·Clinical and Experimental Dermatology·E SarifakiogluF Can
Sep 15, 2011·Mycoses·Timo R HammerDirk Hoefer
Oct 19, 2013·International Journal of Dermatology·Gülgün YenişehirliYunus Bulut
Apr 29, 2015·Phytotherapy Research : PTR·Zhi-Jian LiSilafu Aibai
Aug 18, 2009·Revista Da Sociedade Brasileira De Medicina Tropical·Crystiane Rodrigues Araújo MotaMaria do Rosário Rodrigues Silva
Feb 21, 2009·Revista Do Instituto De Medicina Tropical De São Paulo·Crystiane Rodrigues AraújoMaria do Rosário Rodrigues Silva
Nov 21, 2007·Revista Do Instituto De Medicina Tropical De São Paulo·E I NwezeJ I Okafor
Mar 11, 2008·Revista Do Instituto De Medicina Tropical De São Paulo·Emerson Roberto SiqueiraRegina Celia Candido
Mar 13, 2014·Antimicrobial Agents and Chemotherapy·Minh LamElma D Baron
Apr 9, 2014·Antimicrobial Agents and Chemotherapy·Nathan P WiederholdAmir Tavakkol
Jul 21, 2005·The British Journal of Dermatology·M ArabatzisA Katsambas
Mar 16, 2019·Expert Opinion on Drug Delivery·Riya BangiaOm Prakash Katare
Jul 23, 2009·Expert Review of Anti-infective Therapy·Emilia CantónJavier Pemán
Aug 12, 2009·Anais Brasileiros De Dermatologia·Lívia Maria Martins de AlmeidaTerezinha Inez Estivalet Svidzinski
Jul 8, 2018·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·Zahra SalehiMehdi Razzaghi-Abyaneh
Sep 8, 2018·Journal of Fungi·Chioma I AnekeClaudia Cafarchia
Aug 8, 2020·Frontiers in Microbiology·Anthi-Marina MarkantonatouTimoleon-Achilleas Vyzantiadis
Aug 9, 2020·Infection and Drug Resistance·Mostafa Chadeganipour, Rasoul Mohammadi
Apr 4, 2021·Antibiotics·Chioma Inyang AnekeClaudia Cafarchia

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antifungals

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Antifungals (ASM)

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.