Intra-aortic clusters undergo endothelial to hematopoietic phenotypic transition during early embryogenesis.

PloS One
Chiyo MizuochiDaisuke Sugiyama

Abstract

Intra-aortic clusters (IACs) attach to floor of large arteries and are considered to have recently acquired hematopoietic stem cell (HSC)-potential in vertebrate early mid-gestation embryos. The formation and function of IACs is poorly understood. To address this issue, IACs were characterized by immunohistochemistry and flow cytometry in mouse embryos. Immunohistochemical analysis revealed that IACs simultaneously express the surface antigens CD31, CD34 and c-Kit. As embryos developed from 9.5 to 10.5 dpc, IACs up-regulate the hematopoietic markers CD41 and CD45 while down-regulating the endothelial surface antigen VE-cadherin/CD144, suggesting that IACs lose endothelial phenotype after 9.5 dpc. Analysis of the hematopoietic potential of IACs revealed a significant change in macrophage CFC activity from 9.5 to 10.5 dpc. To further characterize IACs, we isolated IACs based on CD45 expression. Correspondingly, the expression of hematopoietic transcription factors in the CD45(neg) fraction of IACs was significantly up-regulated. These results suggest that the transition from endothelial to hematopoietic phenotype of IACs occurs after 9.5 dpc.

References

Nov 1, 1995·Anatomy and Embryology·J A Garcia-PorreroF Dieterlen-Lièvre
Apr 16, 1996·Proceedings of the National Academy of Sciences of the United States of America·Q WangN A Speck
Aug 13, 1998·Developmental and Comparative Immunology·J A Garcia-PorreroI E Godin
Aug 3, 2002·Nature Reviews. Immunology·Isabelle Godin, Ana Cumano
Mar 13, 2003·Development, Growth & Differentiation·Stuart T FraserShin-Ichi Nishikawa
Oct 6, 2004·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Muktadir S HossainKazuhisa Sekimizu
Dec 30, 2004·Proceedings of the National Academy of Sciences of the United States of America·Julien Y BertrandAna Cumano
May 21, 2005·The International Journal of Developmental Biology·Thierry JaffredoCécile Drevon
Sep 6, 2005·Experimental Hematology·Manuela Tavian, Bruno Péault
Nov 30, 2005·The Journal of Experimental Medicine·Azusa MatsubaraHiromitsu Nakauchi
Jan 26, 2006·Stem Cells and Development·Daisuke SugiyamaKohichiro Tsuji
Feb 14, 2006·Trends in Cardiovascular Medicine·Daisuke Sugiyama, Kohichiro Tsuji
May 23, 2006·Trends in Cardiovascular Medicine·Françoise Dieterlen-LièvreThierry Jaffredo
Sep 14, 2006·Development·Hanna K A Mikkola, Stuart H Orkin
Jan 22, 2008·Nature Immunology·Elaine Dzierzak, Nancy A Speck
Dec 2, 2008·Cell Stem Cell·Ann C ZoveinM Luisa Iruela-Arispe
Feb 16, 2010·Nature·Jean-Charles BoissetCatherine Robin
Apr 7, 2010·Cell Stem Cell·Gemma SwiersMarella F T R de Bruijn
Jun 1, 2011·The Journal of Experimental Medicine·Stanislav RybtsovAlexander Medvinsky

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Citations

Jun 12, 2012·Stem Cell Reviews and Reports·Tomoko Inoue-YokooDaisuke Sugiyama
Jul 16, 2013·Differentiation; Research in Biological Diversity·Hyun Jung LeePamela L Wenzel
Feb 6, 2016·Cellular and Molecular Life Sciences : CMLS·Emily Gritz, Karen K Hirschi
Dec 29, 2012·The International Journal of Biochemistry & Cell Biology·Veronica I AntasStuart T Fraser
Jun 21, 2014·Experimental Hematology·Aldo Ciau-UitzRoger Patient
Jun 11, 2014·Developmental Cell·Natasha AroraGeorge Q Daley
May 17, 2017·Critical Reviews in Biochemistry and Molecular Biology·Jenna RichterKarl Willert
Apr 13, 2017·Blood Research·Ayako YumineDaisuke Sugiyama
Mar 26, 2014·Molecular and Cellular Biology·Ikuo NobuhisaTetsuya Taga
Jun 26, 2013·Stem Cell Research & Therapy·Wai Feng LimDaisuke Sugiyama
Nov 17, 2016·Stem Cells and Development·Daisuke SugiyamaAlistair R R Forrest
Jul 8, 2021·Cellular and Molecular Life Sciences : CMLS·Honghu LiHe Huang
Dec 22, 2012·Stem Cell Research & Therapy·Kasem Kulkeaw, Daisuke Sugiyama

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Methods Mentioned

BETA
flow cytometry
confocal microscopy
Fluorescence
PCR

Software Mentioned

FlowJo

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