Intra-arterial catheter system to repeatedly deliver mesenchymal stem cells in a rat renal failure model

Clinical and Experimental Nephrology
Yuichi KatsuokaTakashi Yokoo

Abstract

Mesenchymal stem cell therapy in renal failure is rarely used because of low rates of cell engraftment after systemic delivery. Repeated intra-arterial cell administration may improve results; however, no current delivery method permits repeated intra-arterial infusions in a rat model. In this study, we developed an intra-arterial delivery system for repeated stem cell infusion via the aorta, catheterizing the left femoral artery to the suprarenal aorta under fluoroscopic guidance in rats with adenosine-induced renal failure. First, we compared our intra-arterial catheter system (C group, n = 3) with tail vein injection (V group, n = 3) for engraftment efficacy, using mesenchymal stem cells from luciferase transgenic rats. Rats were infused with the cells and euthanized the following day; we performed cell-tracking experiments using a bioluminescence imaging system to assess the distribution of the infused cells. Second, we assessed the safety of the system over a 30-day period in a second group of six rats receiving infusions every 7 days. Cells infused through our delivery system efficiently engrafted into the kidney, compared with peripheral venous infusion. In five of the six rats in the safety study, the delivery system re...Continue Reading

References

Mar 17, 2007·Transplantation Proceedings·S SchrepferM P Pelletier
May 16, 2008·American Journal of Physiology. Renal Physiology·Florian TögelChristof Westenfelder
Jun 21, 2008·Stem Cells and Development·Florian TögelChristof Westenfelder
Jan 28, 2009·Nature Reviews. Immunology·Antonio UccelliVito Pistoia
Jul 23, 2011·Nephrology·Andrea F Wise, Sharon D Ricardo
Sep 10, 2011·Fibrogenesis & Tissue Repair·William R Otto, Nicholas A Wright
Sep 1, 2011·Kidney International Supplements·Christof Westenfelder, Florian E Togel

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Citations

Jun 3, 2021·Cells·Michael NamestnikovBenjamin Dekel

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