PMID: 2103305Jan 1, 1990Paper

Intra-peritoneal administration of interleukin-1 beta induces impaired insulin release from the perfused rat pancreas

Autoimmunity
L D WogensenJ Nerup

Abstract

Previous studies have demonstrated a stimulatory effect of interleukin-1 beta (IL-1 beta) on insulin and glucagon release from the perfused rat pancreas, accompanied by selective lysis of 20% of beta-cells as assessed by electronmicroscopy. However, we have not observed an inhibitory action of IL-1 beta on insulin release from the perfused pancreas as shown for isolated islets. To test whether periodical exposure of the endocrine pancreas to circulating IL-1 beta in vivo affects insulin release from the intact perfused pancreas, rats were treated with daily intraperitoneal injections of 4 micrograms IL-1 beta/kg or saline for 5 days. On day 5 the pancreata were isolated 2 h after the last injection and perfused from 0 to 72 min with 11 mmol/l D-glucose and from 72 to 84 min with 20 mmol/l D-glucose. Saline or IL-1 beta was added from 12 to 72 min. In pancreata from animals pre-treated with IL-1 beta glucose-stimulated as well as IL-1 beta potentiated glucose-stimulated insulin release was almost completely abolished. Furthermore, a decline in insulin release was observed at 11 mmol/l D-glucose, in contrast to an increase in insulin release in controls. The total extractable insulin content in pancreata from IL-1 beta pre-treate...Continue Reading

References

Sep 18, 1989·Diabetes Research and Clinical Practice·Y WangB Draznin
Jul 1, 1986·Acta Endocrinologica·A SvenningsenA Lernmark
Jul 1, 1984·Metabolism: Clinical and Experimental·A Svenningsen, V Bonnevie-Nielsen

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Citations

May 6, 2011·The Journal of Clinical Endocrinology and Metabolism·Mihai G Netea, Charles A Dinarello
Mar 1, 1994·Diabetes Research and Clinical Practice·Y Sudo, M Itoh
Dec 1, 1993·Diabetes/metabolism Reviews·T Mandrup-PoulsenJ Nerup

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