Intra-vitreal αB crystallin fused to elastin-like polypeptide provides neuroprotection in a mouse model of age-related macular degeneration

Journal of Controlled Release : Official Journal of the Controlled Release Society
Parameswaran G SreekumarJ Andrew MacKay

Abstract

Age-related macular degeneration (AMD) is the leading cause of severe and irreversible central vision loss, and the primary site of AMD pathology is the retinal pigment epithelium (RPE). Geographic atrophy (GA) is an advanced form of AMD characterized by extensive RPE cell loss, subsequent degeneration of photoreceptors, and thinning of retina. This report describes the protective potential of a peptide derived from the αB crystallin protein using a sodium iodate (NaIO3) induced mouse model of GA. Systemic NaIO3 challenge causes degeneration of the RPE and neighboring photoreceptors, which have similarities to retinas of GA patients. αB crystallin is an abundant ocular protein that maintains ocular clarity and retinal homeostasis, and a small peptide from this protein (mini cry) displays neuroprotective properties. To retain this peptide for longer in the vitreous, mini cry was fused to an elastin-like polypeptide (ELP). A single intra-vitreal treatment by this crySI fusion significantly inhibits retinal degeneration in comparison to free mini cry. While mini cry is cleared from the eye with a mean residence time of 0.4 days, crySI is retained with a mean residence time of 3.0 days; furthermore, fundus photography reveals evide...Continue Reading

Citations

Jul 4, 2020·Expert Opinion on Drug Delivery·Shuying WangW John Kao
Jul 10, 2019·Matrix Biology : Journal of the International Society for Matrix Biology·Arturo Ibáñez-FonsecaJosé Carlos Rodríguez-Cabello
Jul 12, 2019·Matrix Biology : Journal of the International Society for Matrix Biology·Aurélie Le PageTamas Fulop
Oct 28, 2019·Nanomedicine : Nanotechnology, Biology, and Medicine·Zhe LiJohn Andrew MacKay
May 8, 2021·Biomacromolecules·Tianqi NieZhiyu He
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Sep 26, 2021·Journal of Cellular and Molecular Medicine·Tal Carmy-BennunAbigail S Hackam

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