Intracaecal Orthotopic Colorectal Cancer Xenograft Mouse Model

Bio-protocol
Hsin-Wei Liao, Mien-Chie Hung

Abstract

The host microenvironment plays a prominent role in tumor growth, angiogenesis, invasion, metastasis, and response to therapy. Orthotopic tumor model mimics the natural environment of tumor development and provides an effective approach to investigate tumor pathophysiology and develop therapeutic strategies. This protocol describes the technique involving injection of colorectal cancer cell suspension into the intestinal wall of mice to establish an orthotopic colorectal tumor model.

References

Feb 27, 2007·The American Journal of Pathology·James E TalmadgeAvraham Raz
Nov 8, 2008·Journal of Visualized Experiments : JoVE·William TsengEdgar Engleman
Dec 3, 2008·Disease Models & Mechanisms·Ann Richmond, Yingjun Su
Dec 3, 2008·Disease Models & Mechanisms·Aarthi Gopinathan, David A Tuveson
Jan 25, 2014·Journal of Pharmacy & Bioallied Sciences·Thierry F Vandamme
Nov 17, 2015·The Journal of Clinical Investigation·Hsin-Wei LiaoMien-Chie Hung
Nov 4, 2016·Nature Reviews. Cancer·Laurence ZitvogelGuido Kroemer

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Citations

Sep 29, 2019·International Journal of Molecular Sciences·Ivan RanđelovićJózsef Tóvári
May 22, 2020·Journal of Translational Medicine·Takahito SugaseTakami Sato
Dec 11, 2019·Pathology Oncology Research : POR·Andrea Angelo Pierluigi TripodiJózsef Tóvári
Jul 2, 2020·Cancer Management and Research·Wojciech Jelski, Barbara Mroczko
Apr 23, 2020·Animal Models and Experimental Medicine·Rui C OliveiraMaria Filomena Botelho

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