Intracellular accumulation of norfloxacin in Mycobacterium smegmatis.

Antimicrobial Agents and Chemotherapy
S CortiA Cremieux

Abstract

To evaluate the intracellular accumulation of norfloxacin in mycobacteria, two methods were used with Mycobacterium smegmatis. A radiometric method (K. V. Cundy, C. E. Fasching, K. E. Willard, and L. R. Peterson, J. Antimicrob. Chemother. 28:491-497, 1991) was used without great modification, but the fluorometric method (P. G. S. Mortimer and L. J. V. Piddock, J. Antimicrob. Chemother. 28:639-653, 1991) was changed considerably. Indeed, adsorption of the quinolone to the bacterial surface was characterized by measuring the level of accumulation of 0 degree C. Taking into account the adsorption, the pH of the washing buffer was increased from 7.0 to 9.0 to improve the desorption of norfloxacin from the cell surface. Both the fluorometric method, with the technical improvement, and the radiometric method could be used to estimate the intracellular accumulation of norfloxacin, which resulted from the difference between the whole uptake measured at 37 degrees C and the adsorption measured at 0 degrees C. A total of 35 ng of norfloxacin per mg of cells (dry weight) penetrated into the M. smegmatis cell, and the steady state was achieved in 5 min. Use of inhibitors of the proton motive force revealed that transport of norfloxacin was...Continue Reading

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Citations

Feb 1, 1997·International Journal of Antimicrobial Agents·B Heym, S T Cole
Jun 19, 2008·Antimicrobial Agents and Chemotherapy·Olga DanilchankaMichael Niederweis
Oct 17, 2015·The Journal of Antimicrobial Chemotherapy·Caroline M PuleGail E Louw
Apr 29, 1998·Antimicrobial Agents and Chemotherapy·K J WilliamsL J Piddock

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