PMID: 6099561Nov 1, 1984

Intracellular acetylation of desacetyl alpha MSH in the Xenopus laevis neurointermediate lobe

Peptides
M E Goldman, Y P Loh

Abstract

High performance liquid chromatography (HPLC) followed by radioimmunoassay (RIA) of the chromatographic fractions were used to separate and quantify, respectively, the alpha MSH-like peptides stored in the neurointermediate lobe (NIL) of the Xenopus laevis (X. laevis) pituitary gland and released from the X. laevis NIL, in vitro. Immunoreactive (IR) material eluting with a similar HPLC retention time as desacetyl alpha MSH was the major IR peptide in the NIL. Material with a retention time similar to alpha MSH and immunological properties equivalent to alpha MSH was also present in the NIL. However, the retention times of the X. laevis and mammalian alpha MSH-like peptides were not identical, suggesting species difference in these peptides. Following incubation of NILs in the presence of [3H]-acetyl CoA, the X. laevis variant of alpha MSH was the major [3H]-labeled, immunoprecipitable material present. Following an incubation of NILs in the presence of [3H]-amino acids for 21 hours, immunoprecipitable [3H]-alpha MSH was detected in the NILs and the ratio of [3H]-desacetyl alpha MSH to [3H]-alpha MSH was similar to the ratio of IR-desacetyl alpha MSH to IR-alpha MSH. The X. laevis variant of alpha MSH was the major alpha MSH-lik...Continue Reading

References

Jul 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·R E MainsN Ling
Nov 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·J L Roberts, E Herbert
Feb 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·Y P Loh
Dec 1, 1971·Endocrinology·K C Swearingen
Jun 16, 1980·Biochemical and Biophysical Research Communications·Y P LohM Brownstein

Citations

May 1, 1989·Brain Research. Molecular Brain Research·S JégouH Vaudry
Jan 1, 1986·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·E FolleniusA Meunier
Nov 1, 1986·Brain Research Bulletin·B M Verburg-van KemenadeA G Driessen
Jan 1, 1990·Comparative Biochemistry and Physiology. C, Comparative Pharmacology and Toxicology·I D Van ZoestE W Roubos
May 31, 1993·Annals of the New York Academy of Sciences·N V BergasaE A Jones
Nov 1, 1987·General and Comparative Endocrinology·R M DoresM E Rothenberg

Related Concepts

Acetylation
Corticotropin
ACTH (1-13)NH2
Metazoa
Intropin
Melanocyte stimulating hormone
Peptide Fragments
Pituitary Stalk
Xenopus laevis
In Vitro [Publication Type]

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