PMID: 8595868Jan 15, 1996Paper

Intracellular acidification does not account for inhibition of Saccharomyces cerevisiae growth in the presence of ethanol

FEMS Microbiology Letters
M F Rosa, Isabel Sá-Correia

Abstract

Intracellular acidification has been considered one of a number of mechanisms underlying the inhibition of growth and fermentation by ethanol in yeast. However, most of the studies on the effect of ethanol on yeast intracellular pH (pHi) were carried out by using unadapted cells to which ethanol was added. In this paper we show that the pHi of exponential cells of Saccharomyces cerevisiae IGC 3507 III grown in a medium with glucose and inhibitory concentrations of ethanol only decreased to values below those in unstressed cells (6.9) for concentrations equal to or above 7% (v/v). Only at these supracritical levels (7-10% (v/v)) was pH homeostasis in ethanol-adapted yeast affected. This is consistent with the significant increase of plasma membrane permeability and decrease of plasma membrane H(+)-ATPase in comparison with the corresponding values in unstressed cells. These deleterious effects were only observed with those high concentrations of toxin. These results indicate that intracellular acidification does not account for inhibition of yeast growth in the presence of ethanol. In fact, growth was inhibited by ethanol concentrations (3-6% (v/v)) that did not lead to the decrease of pHi. Furthermore, even for supracritical co...Continue Reading

Citations

Jan 9, 2013·Applied Biochemistry and Biotechnology·Jinjing WangQi Li
May 1, 2013·Antimicrobial Agents and Chemotherapy·Catarina CostaMiguel C Teixeira
Jul 28, 2009·Applied and Environmental Microbiology·Miguel C TeixeiraIsabel Sá-Correia
Mar 5, 2005·Applied and Environmental Microbiology·Minoska ValliDiethard Mattanovich
Mar 1, 2014·The Journal of Antimicrobial Chemotherapy·Catarina CostaMiguel C Teixeira
May 14, 2010·Applied Microbiology and Biotechnology·Menggen Ma, Z Lewis Liu
Mar 1, 2016·Frontiers in Microbiology·Elisabet Navarro-TapiaRoberto Pérez-Torrado
Jul 25, 2007·FEMS Microbiology Reviews·Brian R GibsonKatherine A Smart
May 7, 2009·FEMS Yeast Research·Christopher SnowdonGeorge van der Merwe
Jun 17, 2016·Molecular Biology of the Cell·Petri-Jaan LahtveeJens Nielsen
Mar 15, 2018·AMB Express·Yingjie MiaoPeifang Weng
Apr 4, 2014·Biological & Pharmaceutical Bulletin·Pengyao ZhangTakuo Ogihara
Jan 28, 2019·Reviews on Environmental Health·Bodjui Olivier AboQunhui Wang

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