Intracellular adaptations of glutathione content in Cucurbita pepo L. induced by treatment with reduced glutathione and buthionine sulfoximine

Protoplasma
B ZechmannG Zellnig

Abstract

The intracellular effects of GSH (reduced glutathione) and BSO (buthionine sulfoximine) treatment on glutathione content were investigated with immunogold labeling in individual cellular compartments of Cucurbita pepo L. seedlings. Generally, GSH treatment led to increased levels of glutathione in roots and leaves (up to 3.5-fold in nuclei), whereas BSO treatment significantly decreased glutathione content in all organs. Transmission electron microscopy revealed that glutathione levels in mitochondria, which showed the highest glutathione labeling density of all compartments, remained generally unaffected by both treatments. Since glutathione within mitochondria is involved in the regulation of cell death, these results indicate that high and stable levels of glutathione in mitochondria play an important role in cell survival strategies. BSO treatment significantly decreased glutathione levels (1) in roots by about 78% in plastids and 60.8% in the cytosol and (2) in cotyledons by about 55% in the cytosol and 38.6% in plastids. After a short recovery period, glutathione levels were significantly increased in plastids and the cytosol of root tip cells (up to 3.7-fold) and back to control values in cotyledons. These results indica...Continue Reading

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Citations

Feb 27, 2010·Protoplasma·Bernd Zechmann, Maria Müller
Mar 30, 2010·Protoplasma·Bernd ZechmannHrvoje Fulgosi
Mar 29, 2011·BMC Plant Biology·Bernd ZechmannScott D Russell
Apr 11, 2012·International Journal of Molecular Sciences·Marijke JozefczakAnn Cuypers
Jul 14, 2010·Ecotoxicology and Environmental Safety·Maria DrazkiewiczZbigniew Krupa
Jul 7, 2009·Journal of Plant Physiology·Nieves Fernandez-GarcíaEnrique Olmos
Jun 6, 2008·Plant Physiology and Biochemistry : PPB·Anna B OhlssonMaria Greger
Dec 26, 2012·Micron : the International Research and Review Journal for Microscopy·Barbara E KofflerBernd Zechmann
Nov 5, 2014·Frontiers in Plant Science·Bernd Zechmann
May 19, 2012·International Journal of Molecular Sciences·András SzarkaGábor Bánhegyi
Nov 5, 2011·Journal of Integrative Plant Biology·Barbara Eva KofflerBernd Zechmann
Dec 15, 2020·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Rabbul Ibne A Ahad, Mayashree B Syiem

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