PMID: 11607505Jan 3, 1995Paper

Intracellular Agrobacterium can transfer DNA to the cell nucleus of the host plant

Proceedings of the National Academy of Sciences of the United States of America
J EscuderoB Hohn

Abstract

Agrobacterium tumefaciens is a Gram-negative, soil-borne bacterium responsible for the crown gall disease of plants. The galls result from genetic transformation of plant cells by the bacteria. Genes located on the transferred DNA (T-DNA), which is part of the large tumor-inducing (Ti) plasmid of Agrobacterium, are integrated into host plant chromosomes and expressed. This transfer requires virulence (vir) genes that map outside the T-DNA on the Ti plasmid and that encode a series of elaborate functions that appear similar to those of interbacterial plasmid transfer. It remains a major challenge to understand how T-DNA moves from Agrobacterium into the plant cell nucleus, in view of the complexity of obstacles presented by the eukaryotic host cell. Specific anchoring of bacteria to the outer surface of the plant cell seems to be an important prelude to the mobilization of the T-DNA/protein complex from the bacterial cell to the plant cell. However, the precise mode of infection is not clear, although a requirement of wounded cells has been documented. By using a microinjection approach, we show here that the process of T-DNA transfer from the bacteria to the eukaryotic nucleus can occur entirely inside the plant cell. Such tran...Continue Reading

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Citations

Jul 1, 1996·World Journal of Microbiology & Biotechnology·K Weising, G Kahl
Mar 12, 2004·Annual Review of Plant Physiology and Plant Molecular Biology·Stanton B. Gelvin
Mar 27, 2010·Annual Review of Phytopathology·Stanton B Gelvin
Jan 28, 2014·Biochemistry. Biokhimii︠a︡·M I Chumakov
Feb 17, 1998·Applied and Environmental Microbiology·H MarcinekM Gauer
Feb 1, 1997·Experimental Cell Research·D A Dean

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