Intracellular alkalinization augments capacitative Ca2+ entry in vascular smooth muscle cells

Journal of Cardiovascular Pharmacology
Ichiro WakabayashiYoko Sotoda

Abstract

Agonist-induced Ca2+ influx of vascular smooth muscle cells is thought to be triggered by depletion of intracellular Ca2+ stores. This study investigated the effects of intracellular alkalinization on capacitative Ca2+ entry in A7r5 rat aortic smooth muscle cells. Intracellular alkalinization was induced by NH(4)Cl. Transplasmalemmal Ca2+ influx due to Ca2+ store depletion induced by thapsigargin, which was abolished by pretreatment of the cells with SKF-96365 but not affected by that with verapamil, was significantly increased by pretreatment with NH(4)Cl. Neither 5-hydroxytryptamine-induced inositol monophosphate accumulation nor intracellular Ca2+ release from its stores was affected by NH(4)Cl. These results suggest that intracellular alkalinization acts on the process(es) after depletion of Ca2+ stores and facilitates capacitative Ca2+ entry in vascular smooth muscle cells.

References

Aug 15, 1991·Biochemical and Biophysical Research Communications·I WakabayashiS Yoshimoto
Oct 15, 1990·The Biochemical Journal·J E MerrittT J Rink
Feb 1, 1986·Cell Calcium·J W Putney
Feb 1, 1989·The American Journal of Physiology·M S SiskindJ H Schwartz
Jan 1, 1986·The Journal of Membrane Biology·S Grinstein, A Rothstein
Sep 23, 1987·European Journal of Pharmacology·N R DanthuluriR C Deth
Apr 1, 1994·The Journal of General Physiology·U Klöckner, G Isenberg
Apr 1, 1996·General Pharmacology·H TanakaE Kakishita
Nov 1, 1996·Pflügers Archiv : European journal of physiology·R NitschkeR Greger
Jun 5, 1998·Trends in Pharmacological Sciences·M TreimanS B Christensen

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Citations

Jun 13, 2015·Pulmonary Circulation·John Huetsch, Larissa A Shimoda
Nov 14, 2006·European Journal of Pharmacology·Carolina A Restini, Lusiane M Bendhack
Feb 2, 2006·Journal of Vascular Research·Ichiro WakabayashiKlaus Groschner
Feb 23, 2021·Pulmonary Circulation·Guogu LiuAiguo Dai

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