Intracellular amino acids and protein synthesis in Hela cells

Molecular Biology Reports
W J van Venrooij, L van Loon-Klaassen


(1) When the intracellular amino acid pool is prelabelled and subsequently chased in non-radioactive medium, the radioactivity of the amino acid pool is not found to have been incorporated into protein. (2) Leucine transport into Hela cells is reduced in the presence of 10 mM valine in the medium. This results in a lower specific radioactivity of leucine in the intracellular amino acid pool. However, neither the overall rate of protein synthesis nor the incorporation of radioactive leucine into protein is affected. From these experiments it is concluded that incoming amino acids entering the intracellular amino acid pool are not used for de novo synthesis of protein.


Nov 7, 1972·European Journal of Biochemistry·W J Van VenrooijM T Jansen
Jun 23, 1973·Biochimica Et Biophysica Acta·W J van VenrooijM F Kramer
Apr 12, 1972·Biochimica Et Biophysica Acta·D H Alpers, S O Thier
Jul 1, 1971·Journal of Biochemistry·K TsukadaI Lieberman
Feb 1, 1968·Experimental Cell Research·W E Berg
Nov 28, 1967·Biochimica Et Biophysica Acta·J C Gan, H Jeffay
Aug 19, 1961·Biochimica Et Biophysica Acta·D M KIPNISE HELMREICH

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May 1, 1978·Biological Reviews of the Cambridge Philosophical Society·R M Case
Sep 1, 1980·Journal of Neurochemistry·B E DwyerC G Wasterlain
Dec 16, 1974·European Journal of Biochemistry·W J Van VenrooijL Van Loon-Klaassen
Jun 1, 1976·Wilhelm Roux's Archives of Developmental Biology·D A McGregor, B G Loughton
May 22, 1984·Biochimica Et Biophysica Acta·S A Austin, M J Clemens

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