PMID: 13563545Jul 25, 1958Paper

Intracellular amounts of nucleic acids and protein during pollen grain growth in Tradescantia

The Journal of Biophysical and Biochemical Cytology
J W WOODARD

Abstract

The rapid growth, large organelles, and synchronous development of T. paludosa pollen grains make them ideal subjects for cytochemical analysis. A microphotometric study of the nucleoli, chromosomes, and cytoplasm fixed at daily intervals during pollen grain maturation indicated that: 1. DNA (Feulgen) synthesis in the generative nucleus occurred during the first third of interphase, while the DNA content of the vegetative nucleus remained unchanged. 2. Throughout development, changes in RNA (azure B) content, in general, paralleled changes in protein (NYS(1), Millon) content in each organelle of the vegetative cell. Initially, the RNA and protein of all organelles increased up to mid interphase, when chromosomal and nucleolar fractions began to decline despite a continued increase in cytoplasmic RNA and protein. At least 24 hours before anthesis, the vegetative nucleolus had disappeared and chromosomal protein and RNA of the vegetative nucleus were apparently in rapid decline. Such a system offered an opportunity to study the role of the nucleus, especially the nucleolus, in RNA and protein metabolism in the cytoplasm, by noting what cytoplasmic processes could and could not continue at a time when nuclear mechanisms were absen...Continue Reading

References

Dec 1, 1955·Experimental Cell Research·M J MOSES, J H TAYLOR
Mar 25, 1956·The Journal of Biophysical and Biochemical Cytology·G E PALADE, P SIEKEVITZ
Jul 25, 1956·The Journal of Biophysical and Biochemical Cytology·H SWIFT
Jan 1, 1956·Cold Spring Harbor Symposia on Quantitative Biology·A E MIRSKYV G ALLFREY
Nov 1, 1950·Proceedings of the National Academy of Sciences of the United States of America·H SWIFT

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Citations

Apr 1, 1970·Developmental Biology·J P Mascarenhas, E Bell
May 15, 1961·Proceedings of the National Academy of Sciences of the United States of America·D M PRESCOTT, R F KIMBALL
Oct 1, 1959·The Journal of Biophysical and Biochemical Cytology·E RASCH, J W WOODARD
Aug 1, 1960·Experimental Cell Research·A C LONGWELL, G SVIHLA
Aug 1, 1967·Biological Reviews of the Cambridge Philosophical Society·I K Vasil
Jan 21, 1970·Biochimica Et Biophysica Acta·G Wever, S T Takats

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