PMID: 5921539May 1, 1966

Intracellular and extracellular compartments of mammalian central nervous tissue

The Journal of Physiology
A Ames, F B Nesbett

Abstract

1. Isolated rabbit retina was used as a prototype of grey matter to study the partition of water and electrolytes between the intracellular and extracellular phase. Previously published morphologic, chemical, and functional evidence has shown that it can be maintained in vitro in a nearly physiological state.2. Following equilibration with mannitol or inulin, retinas were eluted in isotonic tris acetate at 0 degrees C, and measurements were made of the rates at which K(+), Na(+), Cl(-) and inulin or mannitol diffused from the tissue.3. K(+) was eluted slowly according to a single exponential decay. The Na(+) and Cl(-) elution curves demonstrated two phases which could be dissected into a rapid, multicomponent regression superimposed upon a slow exponential decrease.4. The volume of distribution of the readily elutable Na(+) equalled that of the readily elutable Cl(-) and corresponded closely to the volume of distribution of mannitol and inulin. On the basis of these and previously published data, the interstitial fluid was estimated to constitute 31% (w/w) of rabbit retina and 22% of rabbit brain.5. The composition of the extracellular fluid of retina resembled closely that of the medium in which the tissue had been previously ...Continue Reading

Citations

Aug 1, 1980·Neurochemical Research·I M Vivas, B D Drujan
Apr 1, 1980·Pharmacology, Biochemistry, and Behavior·A van HarreveldJ L Davis
Jan 1, 1982·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·B Bauer
Nov 1, 1976·Journal of Neurochemistry·A AmesF B Nesbett
Sep 1, 1983·The American Journal of Emergency Medicine·A Ames
Feb 14, 1983·Brain Research·M C Trachtenberg, D J Packey
Dec 14, 2007·Neurobiology of Aging·Handi ZhangDai Zhang
Jan 1, 1968·Journal of Neurochemistry·L Hertz
Jun 8, 2018·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Caitlin M Davis, Martin Gruebele
Jan 1, 1970·Pflügers Archiv : European journal of physiology·A BaethmannW Brendel
Jul 1, 1979·Journal of Neurobiology·A van Harreveld
Jan 1, 1986·Journal of Neuroscience Research·M C Trachtenberg, K M Hokanson
Mar 1, 1983·Stroke; a Journal of Cerebral Circulation·A Ames, F B Nesbett
Apr 1, 1979·Journal of Neurochemistry·P V Sarthy, D M Lam
Jul 31, 1973·Pflügers Archiv : European journal of physiology·U Borchard, W Erasmi
Feb 6, 2007·Annals of Biomedical Engineering·Feilim Mac GabhannAleksander S Popel
Nov 22, 1984·Proceedings of the Royal Society of London. Series B, Containing Papers of a Biological Character·R H MaslandC Cassidy
Sep 3, 2020·Journal of Pharmacokinetics and Pharmacodynamics·David Bussing, Dhaval K Shah
Apr 8, 2021·The Journal of Physical Chemistry. B·Caitlin M Davis, Martin Gruebele

Related Concepts

Body Fluids
Cell Membrane Permeability
Chloride Ion Level
Extracellular Space
Inulin
Isotonic Solutions
(L)-Mannitol
Nerve Tissue
Potassium
Retina

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