Intracellular and plasma cytokine profile in neonates born to non-atopic parents: the impact of breast feeding

European Journal of Pediatrics
Florence Kanakoudi-TsakalidouGeorgios Kremenopoulos

Abstract

The aim of this study was to profile the changes in intracellular and plasma cytokines during the neonatal period and evaluate the impact of breast feeding on these parameters. For this purpose, we measured the interleukin (IL)-2 and IL-4 producing CD3+/CD69+ T-cells using flow cytometry and plasma concentrations of interferon (IFN)-gamma and IL-4 using ELISA, in 122 healthy term neonates, aged 6-12 h, born to non-atopic parents, and 25 healthy children aged 1-12 years. A total of 42/122 neonates exclusively breast-fed (BF) and 39/122 formula fed (FF) were studied again on the 30th day of life for the above parameters. Finally, a clinical evaluation for the presence of atopic disease was conducted at 2 years of age. We found that at birth, the percentage of CD3+/CD69+/IL-4+ T-cells (median = 15.8%, range = 4.4%-49%) and plasma concentrations of IL-4 (median = 0.22 pg/ml, range = 0.18-0.25 pg/ml) were significantly higher (P < 0.0001) compared to those of children (median = 1.6%, range = 0.16%-2.7% for CD3+/CD69+/IL-4+ and median = 0.17 pg/ml, range = 0.13-0.26 pg/ml for IL-4), whereas plasma concentrations of IFN-gamma were significantly lower in neonates (median = 0.42 pg/ml, range = 0.3-1.5 pg/ml) than in children (median = 1...Continue Reading

Citations

Feb 17, 2007·Clinical and Experimental Immunology·J HassanW Hall
Dec 17, 2009·Pediatric Allergy and Immunology : Official Publication of the European Society of Pediatric Allergy and Immunology·M RaesJ L Rummens

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